AB155947

Anti-DOK1 antibody [EPR3228(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DOK1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Docking protein 1, Downstream of tyrosine kinase 1, p62(dok), pp62, DOK1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (AB155947)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (AB155947)

Immunofluorescent analysis of K562 cells labeling DOK1 with ab155947 at 1/250 dilution.

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Western blot - Anti-DOK1 antibody [EPR3228(2)] (AB155947)

All lanes:

Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilution

Lane 1:

HuT-78 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3228(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOK1 also known as Docking protein 1 or Downstream of tyrosine kinase 1 is an adaptor protein involved in the signal transduction pathways. This protein weighs approximately 62 kDa and is primarily expressed in hematopoietic cells. While DOK1 lacks enzymatic activity it plays an important role in cellular processes by providing a scaffold for various signaling molecules. It helps to modulate downstream signaling events by interacting with receptor tyrosine kinases and other proteins through its multiple binding domains.

Biological function summary

DOK1 operates as a negative regulator in signal transduction functioning as part of a larger signaling complex. The protein associates with different cellular components forming interactions that influence cell communication and immune response. DOK1 often interacts with proteins like RasGAP and Nck which helps in mediating signals that can affect cell growth differentiation and apoptosis. Its role as a docking station for other proteins allows it to influence cellular homeostasis.

Pathways

DOK1 influences the Ras and MAP kinase signaling pathways. It inhibits the Ras pathway by binding with and recruiting RasGAP leading to the inactivation of Ras an important player in cell proliferation. Additionally DOK1 modulates the JAK/STAT pathway by affecting cytokine signaling and immune regulation. These interactions are significant for controlling cellular responses and maintaining balance in cell signaling networks.

DOK1 is implicated in conditions like leukemia and autoimmune diseases. Aberrant DOK1 expression or malfunction disrupts normal signal transduction contributing to the pathogenesis of these disorders. In leukemia altered DOK1 activity can affect its interactions with proteins such as RasGAP leading to unchecked cell growth and survival. Similarly in autoimmune diseases DOK1's regulatory effect on JAK/STAT signaling might be compromised impacting immune cell function and response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

See full target information Docking protein 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:923 PubMed40826409

2025

Metabolic interplay between endometrial cancer and tumor-associated macrophages: lactate-induced M2 polarization enhances tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodie Liu,Heming Sun,Jing Liang,Huan Yu,Ming Xue,Yuebo Li,Wenhui Li,Ying Xu,Bin Ling,Dingqing Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOK1 antibody [EPR3228(2)]

Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (AB155947)

Western blot - Anti-DOK1 antibody [EPR3228(2)] (AB155947)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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