JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB75741

Anti-DOK1 (phospho Y398) antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal DOK1 phospho Y398 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DOK1 pY398.

View Alternative Names

Docking protein 1, Downstream of tyrosine kinase 1, p62(dok), pp62, DOK1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK1 (phospho Y398) antibody (AB75741)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK1 (phospho Y398) antibody (AB75741)

ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

Western blot - Anti-DOK1 (phospho Y398) antibody (AB75741)
  • WB

Unknown

Western blot - Anti-DOK1 (phospho Y398) antibody (AB75741)

All lanes:

Western blot - Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution

Lane 1:

K562 cell extracts, unstarved

Lane 2:

K562 cell extracts, starved

Predicted band size: 52 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DOK1 pY398. The exact immunogen used to generate this antibody is proprietary information.

Q99704

style
left-column

Alternative Products

Primary Antibodies

AB8112

Anti-DOK1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (AB8112)

  • 5
  • 1
Primary Antibodies

AB155947

Anti-DOK1 antibody [EPR3228(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (AB155947)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB191606)

  • 5
  • 2
Primary Antibodies

AB45143

Anti-CD32B antibody [EP888Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (AB45143)

  • 0
  • 0
Primary Antibodies

AB72385

Anti-GPR177/WLS antibody

Western blot - Anti-GPR177/WLS antibody (AB72385)

  • 0
  • 0
Primary Antibodies

AB140795

Anti-GPR91 antibody - Cytoplasmic domain

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR91 antibody - Cytoplasmic domain (AB140795)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab75741 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOK1 also known as Docking protein 1 or Downstream of tyrosine kinase 1 is an adaptor protein involved in the signal transduction pathways. This protein weighs approximately 62 kDa and is primarily expressed in hematopoietic cells. While DOK1 lacks enzymatic activity it plays an important role in cellular processes by providing a scaffold for various signaling molecules. It helps to modulate downstream signaling events by interacting with receptor tyrosine kinases and other proteins through its multiple binding domains.
Biological function summary

DOK1 operates as a negative regulator in signal transduction functioning as part of a larger signaling complex. The protein associates with different cellular components forming interactions that influence cell communication and immune response. DOK1 often interacts with proteins like RasGAP and Nck which helps in mediating signals that can affect cell growth differentiation and apoptosis. Its role as a docking station for other proteins allows it to influence cellular homeostasis.

Pathways

DOK1 influences the Ras and MAP kinase signaling pathways. It inhibits the Ras pathway by binding with and recruiting RasGAP leading to the inactivation of Ras an important player in cell proliferation. Additionally DOK1 modulates the JAK/STAT pathway by affecting cytokine signaling and immune regulation. These interactions are significant for controlling cellular responses and maintaining balance in cell signaling networks.

DOK1 is implicated in conditions like leukemia and autoimmune diseases. Aberrant DOK1 expression or malfunction disrupts normal signal transduction contributing to the pathogenesis of these disorders. In leukemia altered DOK1 activity can affect its interactions with proteins such as RasGAP leading to unchecked cell growth and survival. Similarly in autoimmune diseases DOK1's regulatory effect on JAK/STAT signaling might be compromised impacting immune cell function and response.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
See full target information DOK1 pY398
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed36979906

2023

Mechanotransduction Impairment in Primary Fibroblast Model of Krabbe Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Mezzena,Ambra Del Grosso,Roberto Maria Pellegrino,Husam B R Alabed,Carla Emiliani,Ilaria Tonazzini,Marco Cecchini
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com