JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131488

Anti-DOK2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal DOK2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Docking protein 2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), DOK2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-DOK2 antibody (AB131488)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DOK2 antibody (AB131488)

Immunofluorescent analysis of methanol-fixed HeLa cells labelling DOK2 with ab131488 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (AB131488)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (AB131488)

Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling DOK2 with ab131488 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

Western blot - Anti-DOK2 antibody (AB131488)
  • WB

Unknown

Western blot - Anti-DOK2 antibody (AB131488)

All lanes:

Western blot - Anti-DOK2 antibody (ab131488) at 1/500 dilution

All lanes:

Jurkat cell extract

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Docking protein 2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60496

style
left-column

Alternative Products

Primary Antibodies

AB131461

Anti-DOK2 (phospho Y299) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (AB131461)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB33770

Anti-SHC antibody [EP332Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (AB33770)

  • 0
  • 0
Primary Antibodies

AB32078

Anti-Src (phospho Y529) antibody [Y232]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Src (phospho Y529) antibody [Y232] (AB32078)

  • 5
  • 2
Primary Antibodies

AB317439

Anti-14-3-3 zeta antibody [EPR27044-19] - BSA and Azide free

Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody [EPR27044-19] - BSA and Azide free (AB317439)

  • 0
  • 0
Primary Antibodies

AB248822

Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] - BSA and Azide free (AB248822)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab131488 was purified by affinity chromatography using epitope specific peptide.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOK2 also known as Docking Protein 2 or p56dok-2 is an adaptor protein with a molecular weight of approximately 56 kDa. It is expressed in hematopoietic cells and is found in various tissues including the spleen thymus and bone marrow. DOK2 acts mechanically by mediating signal transduction processes and interacting with several receptors particularly those involved in immune response.
Biological function summary

Within the cellular environment DOK2 plays an important role in the negative regulation of cell signaling pathways. It acts as a part of a signaling complex where it inhibits the Ras pathway by interacting with Ras GTPase-activating proteins. This interaction prevents excessive cell proliferation and contributes to maintaining cellular homeostasis. DOK2's inhibitory functions are essential in controlling cell cycle and apoptosis processes.

Pathways

DOK2 operates as a negative regulator in key signaling pathways such as the epidermal growth factor receptor (EGFR) pathway and the T cell receptor (TCR) signaling pathway. In the TCR pathway it associates with proteins like SHIP and SHC thereby playing a part in modulating T cell activation and signal progression. In these pathways DOK2’s presence prevents overactivation that could otherwise lead to pathological conditions.

Aberrant DOK2 function has been linked to the development of certain types of leukemia such as chronic myeloid leukemia (CML) where it may play a role in leukemic cell survival. Altered DOK2 expression also shows connection to autoimmune diseases potentially through dysregulation of immune cell signaling. An interaction with proteins like BCR-ABL in CML illustrates DOK2’s involvement in disease states making it an important focus for research in targeted therapies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).
See full target information Docking protein 2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:374 PubMed41044623

2025

THBS1 regulates the function and insulin sensitivity of HTR8/SVneo cells treated with high glucose through the RhoA/ROCK1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuerong Chai,Jie Tang,Aimin Liu,Qin Zhou
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com