AB131461

Anti-DOK2 (phospho Y299) antibody

Name: Anti-DOK2 (phospho Y299) antibody (ab131461) | Abcam
Brand: Abcam Limited
SKU: AB131461
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DOK2 phospho Y299 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), DOK2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (AB131461)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (AB131461)

Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling DOK2 (phospho Y299) with ab131461 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (AB131461)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (AB131461)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling DOK2 (phospho Y299) with ab131461 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

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Western blot - Anti-DOK2 (phospho Y299) antibody (AB131461)

All lanes:

Western blot - Anti-DOK2 (phospho Y299) antibody (ab131461) at 1/500 dilution

Lane 1:

K562 cell extracts with blocking peptide

Lane 2:

K562 cell extracts

Predicted band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131461 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOK2 also known as Docking Protein 2 or p56dok-2 is an adaptor protein with a molecular weight of approximately 56 kDa. It is expressed in hematopoietic cells and is found in various tissues including the spleen thymus and bone marrow. DOK2 acts mechanically by mediating signal transduction processes and interacting with several receptors particularly those involved in immune response.

Biological function summary

Within the cellular environment DOK2 plays an important role in the negative regulation of cell signaling pathways. It acts as a part of a signaling complex where it inhibits the Ras pathway by interacting with Ras GTPase-activating proteins. This interaction prevents excessive cell proliferation and contributes to maintaining cellular homeostasis. DOK2's inhibitory functions are essential in controlling cell cycle and apoptosis processes.

Pathways

DOK2 operates as a negative regulator in key signaling pathways such as the epidermal growth factor receptor (EGFR) pathway and the T cell receptor (TCR) signaling pathway. In the TCR pathway it associates with proteins like SHIP and SHC thereby playing a part in modulating T cell activation and signal progression. In these pathways DOK2’s presence prevents overactivation that could otherwise lead to pathological conditions.

Aberrant DOK2 function has been linked to the development of certain types of leukemia such as chronic myeloid leukemia (CML) where it may play a role in leukemic cell survival. Altered DOK2 expression also shows connection to autoimmune diseases potentially through dysregulation of immune cell signaling. An interaction with proteins like BCR-ABL in CML illustrates DOK2’s involvement in disease states making it an important focus for research in targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).

See full target information Docking protein 2 phospho Y299

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 119:10338-10350 PubMed30171710

2018

Characterization of potential TRPP2 regulating proteins in early Xenopus embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Mélinée Futel,Ronan Le Bouffant,Isabelle Buisson,Muriel Umbhauer,Jean-François Riou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOK2 (phospho Y299) antibody

Immunocytochemistry/ Immunofluorescence - Anti-DOK2 (phospho Y299) antibody (AB131461)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 (phospho Y299) antibody (AB131461)

Western blot - Anti-DOK2 (phospho Y299) antibody (AB131461)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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