AB168343

Anti-DOK5 antibody [EPR9923(B)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal DOK5 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

C20orf180, DOK5, Docking protein 5, Downstream of tyrosine kinase 5, Insulin receptor substrate 6, IRS-6, IRS6

1 Images

Unknown

Western blot - Anti-DOK5 antibody [EPR9923(B)] (AB168343)

All lanes:

Western blot - Anti-DOK5 antibody [EPR9923(B)] (ab168343) at 1/10000 dilution

Lane 1:

IM9 lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9923(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOK5 also known as Docking Protein 5 is a member of the DOK family of intracellular adapter proteins. It has an approximate mass of 50 kDa. DOK5 primarily expresses in skeletal muscle and the nervous system including the brain and spinal cord. This protein facilitates signal transduction by acting as an adapter or scaffold for the assembly of signaling complexes integrating extracellular signals to downstream pathways.

Biological function summary

DOK5 plays a role in regulating cellular growth differentiation and apoptosis. It does not generally function as part of a larger complex but forms interactions that modulate signaling pathways. These functions depend on the ability of DOK5 to bind with key receptor tyrosine kinases including insulin and neurotrophin receptors. Its expression pattern and activities point to essential roles in muscle homeostasis and neuronal signaling.

Pathways

DOK5 is integral to the insulin signaling and neurotrophin signaling pathways. In relation to the insulin signaling pathway DOK5 interacts closely with insulin receptors and mediates downstream effects that influence glucose metabolism. The protein also forms part of the neurotrophin signaling pathway where it affects neuronal survival and differentiation in conjunction with associated proteins like TrkB and TrkC receptors.

DOK5 associates with conditions such as insulin resistance and neurodevelopmental disorders. Alterations in DOK5 expression or function can contribute to aberrant insulin signaling potentially leading to type 2 diabetes. Additionally its role in the nervous system links DOK5 to neurodevelopmental disorders with connections to proteins such as TrkB which may further elucidate its involvement in neuronal defects.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.

See full target information DOK5

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:193 PubMed40268915

2025

High-fat diet promotes lipotoxicity in the podocytes of uninephrectomized mice: a targeted lipidomics and kidney podocyte-specific analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Se-Hyun Oh,You-Jin Kim,Subin Bae,Hee-Yeon Jung,So-Young Park,Jeong-Hoon Lim,Jang-Hee Cho,Chan-Duck Kim,Sun-Hee Park,Tae-Hwan Kwon,Yong-Jin Kim,Kwang-Hyeon Liu,Yong-Lim Kim

Journal of musculoskeletal & neuronal interactions 22:113-122 PubMed35234166

2022

Dok5 regulates proliferation and differentiation of osteoblast via canonical Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Junguo Wu,Yueming Yu,Haoran Li,Shiwei Sun,Tieqi Zhang,Minghai Wang

Journal of cellular physiology 234:8873-8887 PubMed30515784

2018

MicroRNA-141-3p inhibits retinal neovascularization and retinal ganglion cell apoptosis in glaucoma mice through the inactivation of Docking protein 5-dependent mitogen-activated protein kinase signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Qiong Zhang,Hao Cui,Yong-Bin Yu,Huan-Qi Shi,Yuan Zhou,Mei-Jiao Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOK5 antibody [EPR9923(B)]

Western blot - Anti-DOK5 antibody [EPR9923(B)] (AB168343)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise