Rabbit Polyclonal DOM3Z antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DXO aa 1-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DXO aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O77932
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Target data
Function
Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA (PubMed:28283058). The NAD-cap is present at the 5'-end of some RNAs and snoRNAs (PubMed:28283058). In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (PubMed:28283058). Preferentially acts on NAD-capped transcripts in response to environmental stress (PubMed:31101919). Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation (By similarity). Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control (By similarity). Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1) (PubMed:29601584). In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA (By similarity). Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs (PubMed:29601584). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates (By similarity). Exhibits decapping activity towards FAD-capped RNAs (PubMed:32374864). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity).
Alternative names
DOM3L, DOM3Z, NG6, DXO, Decapping and exoribonuclease protein, 5'-3' exoribonuclease DXO, Dom-3 homolog Z, NAD-capped RNA hydrolase DXO, DeNADding enzyme DXO
Recommended products
Rabbit Polyclonal DOM3Z antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DXO aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DXO aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O77932
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DOM3Z also known as exosome component 1 or hDOM3Z is a protein involved in RNA processing and degradation. It has a molecular mass of about 28 kDa. This protein is present in various tissues with higher expression levels seen in the heart and skeletal muscle. DOM3Z localizes to the nucleus where it plays an important role in maintaining RNA quality control. The protein acts within the RNA exosome complex which is a multi-protein complex essential for RNA decay.
- Biological function summary
DOM3Z forms part of the RNA exosome complex working in concert with other components to remove defective or unnecessary RNA molecules. This activity is essential for regulating RNA homeostasis and ensuring the fidelity of RNA populations within the cell. By interacting with exosome core components and exosome co-factors DOM3Z facilitates the degradation of a wide range of RNA substrates including messenger RNA and non-coding RNA. The proper function of DOM3Z within the exosome complex assures accuracy in gene expression regulation.
- Pathways
The RNA degradation pathway heavily relies on functions executed by DOM3Z. It interacts with related proteins such as RRP6 and RRP44 within the exosome assembly. Another significant pathway involving DOM3Z is the non-coding RNA processing pathway where its involvement ensures the correct biogenesis and maturation of small nuclear RNAs and small nucleolar RNAs. This involvement of DOM3Z and its associated proteins highlights its central role in maintaining cellular RNA integrity and gene expression equilibrium.
- Associated diseases and disorders
Abnormalities in DOM3Z function correlate with neuromuscular disorders reflecting its higher expression levels in muscle tissues. Mutations or misregulation that affect DOM3Z can also relate to certain types of cancer where disrupted RNA processing leads to uncontrolled cell proliferation. In the context of these conditions DOM3Z interacts with several proteins like DIS3L2 which play roles in disease mechanisms. Understanding and investigating these associations may offer new avenues for therapeutic strategies targeting RNA metabolism disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-DOM3Z antibody (ab152135)
10% SDS PAGE
All lanes: Western blot - Anti-DOM3Z antibody (ab152135) at 1/500 dilution
Lane 1: MOLT4 whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Predicted band size: 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DOM3Z antibody (ab152135)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling DOM3Z with ab152135 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com