AB211535

Anti-DOPA Decarboxylase/DDC antibody [CL2962]

Name: Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535) | Abcam
Brand: Abcam Limited
SKU: AB211535
Price: 520 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal DOPA Decarboxylase/DDC antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDC aa 100-250.

View Alternative Names

AADC, DDC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunohistochemical analysis of human kidney tissue staining DDC with ab211535 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunohistochemical analysis of paraffin-embedded human substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunohistochemical analysis of human fallopian tube tissue staining DDC with ab211535 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunohistochemical analysis of paraffin-embedded rat substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunocytochemistry/Immunofluorescent analysis of rat substantia nigra cells staining DOPA Decarboxylase with ab211535.

Supplier Data

Western blot - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

All lanes:

Western blot - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535) at 1/500 dilution

All lanes:

Human kidney tissue lysate

Predicted band size: 53 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2962

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DDC aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P20711

Epitope

Binds to an epitope located within the peptide sequence MDWLGKMLEL (aa 119-128) as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOPA Decarboxylase also known as DDC or aromatic L-amino acid decarboxylase is an enzyme critical in neurotransmitter production. It has a molecular mass of approximately 54 kDa. DOPA Decarboxylase converts L-DOPA to dopamine and 5-HTP to serotonin. This enzyme is expressed in tissues like the brain liver and kidney with high activity levels in the central nervous system.

Biological function summary

DOPA Decarboxylase plays an essential role in the synthesis of neurotransmitters such as dopamine and serotonin. It functions as part of the enzymatic conversion process and is not typically associated with larger complexes. The availability and activity of DDC directly influence the levels of these neurotransmitters affecting various physiological responses.

Pathways

DOPA Decarboxylase participates in the catecholamine biosynthesis pathway. This pathway is important for regulating body functions like mood motivation and fine motor control. DDC activity connects it with proteins such as tyrosine hydroxylase which provides the precursor L-DOPA for dopamine synthesis. Additionally it is involved in the serotonin biosynthesis pathway alongside tryptophan hydroxylase.

DOPA Decarboxylase shows significant relevance to conditions like Parkinson's disease and depression. Reduced DDC activity results in decreased dopamine levels which contribute to Parkinson’s symptoms. In depression altered DDC function affects serotonin levels. Its interaction with proteins like monoamine oxidase also plays a role in these disorders due to their involvement in neurotransmitter metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin.

See full target information DDC

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 19:1507747 PubMed40040846

2025

Dezocine modulates the reinstatement of conditioned place preference in morphine-dependent rats via the dopamine reward circuitry.

Applications

Unspecified application

Species

Unspecified reactive species

Yan He,Zhi-Sheng Piao,Yi Jia,Hui-Qi Wu,Xiao-Qiang Wang,Wei-Feng Yu,Fei-Xiang Wu

Frontiers in oncology 12:1079446 PubMed36544704

2022

Multi-omics profiles refine L-dopa decarboxylase (DDC) as a reliable biomarker for prognosis and immune microenvironment of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Chang,Jiaqi Su,Chuanyu Li,Aihetaimujiang Anwaier,Wangrui Liu,Wenhao Xu,Yuanyuan Qu,Hailiang Zhang,Dingwei Ye

Pharmacology research & perspectives 8:e00612 PubMed32567793

2020

Endothelium-derived dopamine modulates EFS-induced contractions of human umbilical vessels.

Applications

Unspecified application

Species

Unspecified reactive species

José Britto-Júnior,David H A Pinheiro,Alberto F O Justo,Guilherme M Figueiredo Murari,Rafael Campos,Fernanda V Mariano,Valéria B de Souza,André A Schenka,Fabiola Z Mónica,Edson Antunes,Gilberto De Nucci

Stem cell reports 12:518-531 PubMed30799274

2019

Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwon Kim,Hyeok Ju Park,Hwan Choi,Yujung Chang,Hanseul Park,Jaein Shin,Junyeop Kim,Christopher J Lengner,Yong Kyu Lee,Jongpil Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOPA Decarboxylase/DDC antibody [CL2962]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Immunocytochemistry/ Immunofluorescence - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Western blot - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (AB211535)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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