AB307813

Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal DOPA Decarboxylase/DDC antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

AADC, Aromatic-L-amino-acid decarboxylase, DDC_HUMAN, DOPA decarboxylase, DOPA decarboxylase (aromatic L-amino acid decarboxylase)

5 Images

Supplier Data

Immunoprecipitation - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

DOPA Decarboxylase/DDC was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 ug with ab307813 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307813 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : mouse brain tissue lysate 10 ug Lane 2 : ab307813 IP in mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307813 in mouse brain whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (ab307813) at 1/30 dilution

All lanes:

ab307813 IP in mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

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Exposure time: 15s

Supplier Data

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Blocking/Dilution buffer : 5% NFDM/TBST. Exposure times : Lane 1 : 15 seconds, Lane 2 : 37 seconds, Lane 3 : 180 seconds. The lane 3 was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (ab307813) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate

Lane 3:

Human adrenal gland tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 50 kDa

true

Supplier Data

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Blocking/Dilution buffer : 5% NFDM/TBST. Negative control : 293T (PMID : 31177555), SK-OV-3. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (ab307813) at 1/1000 dilution

Lane 1:

SH-SY5Y whole cell lysate at 20 µg

Lane 2:

SK-N-BE(2) whole cell lysate at 20 µg

Lane 3:

293T whole cell lysate at 20 µg

Lane 4:

SK-OV-3 whole cell lysate at 20 µg

Lane 5:

PC-12 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Blocking/Dilution buffer : 5% NFDM/TBST. Negative control : spleen(PMID : 18987999). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (ab307813) at 20 µg

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat kidney tissue lysate at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 15 seconds. Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (ab307813) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse DOPA Decarboxylase expression vector containing a his tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 15s

Carrier free

Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24026-205

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOPA Decarboxylase also known as DDC or aromatic L-amino acid decarboxylase is an enzyme critical in neurotransmitter production. It has a molecular mass of approximately 54 kDa. DOPA Decarboxylase converts L-DOPA to dopamine and 5-HTP to serotonin. This enzyme is expressed in tissues like the brain liver and kidney with high activity levels in the central nervous system.

Biological function summary

DOPA Decarboxylase plays an essential role in the synthesis of neurotransmitters such as dopamine and serotonin. It functions as part of the enzymatic conversion process and is not typically associated with larger complexes. The availability and activity of DDC directly influence the levels of these neurotransmitters affecting various physiological responses.

Pathways

DOPA Decarboxylase participates in the catecholamine biosynthesis pathway. This pathway is important for regulating body functions like mood motivation and fine motor control. DDC activity connects it with proteins such as tyrosine hydroxylase which provides the precursor L-DOPA for dopamine synthesis. Additionally it is involved in the serotonin biosynthesis pathway alongside tryptophan hydroxylase.

DOPA Decarboxylase shows significant relevance to conditions like Parkinson's disease and depression. Reduced DDC activity results in decreased dopamine levels which contribute to Parkinson’s symptoms. In depression altered DDC function affects serotonin levels. Its interaction with proteins like monoamine oxidase also plays a role in these disorders due to their involvement in neurotransmitter metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDC_MOUSE

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205]

Immunoprecipitation - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Western blot - Anti-DOPA Decarboxylase/DDC antibody [EPR24026-205] (AB307813)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise