Rabbit Recombinant Monoclonal Dopamine beta Hydroxylase antibody. Suitable for WB, IP, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 12 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
WB | IP | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Tested |
Rat | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/40 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Tris/EDTA Buffer, pH9 (ab93684) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes Tris/EDTA Buffer, pH9 (ab93684) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2000 | Notes Tris/EDTA Buffer, pH9 (ab93684) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.
Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, DBH
Rabbit Recombinant Monoclonal Dopamine beta Hydroxylase antibody. Suitable for WB, IP, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 12 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Dopamine beta Hydroxylase was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab209487 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab209487 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: SH-SY5Y whole cell lysate, 10 μg (Input).
Lane 2: ab209487 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab209487 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)
Predicted band size: 69 kDa
Observed band size: 75 kDa
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Strong cytoplasmic staining on chromaffin cells of human adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 17699566).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking/Dilution buffer: 5% NFDM/TBST.
Negative controls: HeLa (PMID: 7688735; PMID: 9763470, PMID:15585414, PMID: 10037744), C6 (PMID: 9763470, PMID:7798950, PMID: 10037744), HepG2 (PMID:7798950, PMID:11943777, PMID:15585414).
All lanes: Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487) at 1/1000 dilution
Lane 1: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa
Exposure time: 3min
Immunohistochemical analysis of paraffin-embedded human adrenal pheochromocytoma tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on tumor cells of human adrenal pheochromocytoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2: 1 second.
All lanes: Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487) at 1/1000 dilution
Lane 1: Mouse adrenal gland lysate at 10 µg
Lane 2: Rat adrenal gland lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on chromaffin cells of mouse adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on chromaffin cells of rat adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde fixed rat brain (locus coeruleus) tissue. Stained with ab209487 at 1/1000 dilution. Secondary antibody used was Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated at 1/200 dilution. Blocking was done with 1% BSA for 10 minutes at 21°C. The sample was incubated with the primary antibody in 1% BSA w/ 0.01% Azide for 16 hours at 21°C. Antigen retrieval method was heat mediated Citrate pH 6.4.
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