Anti-Dopamine beta Hydroxylase antibody [EPR20385]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on chromaffin cells of human adrenal gland medulla, and negative on adrenal cortex (PMID : 17535872, PMID : 17699566).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Immunohistochemical analysis of paraffin-embedded human adrenal pheochromocytoma tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on tumor cells of human adrenal pheochromocytoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Dopamine beta Hydroxylase was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab209487 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab209487 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : SH-SY5Y whole cell lysate, 10 μg (Input).

Lane 2 : ab209487 IP in SH-SY5Y whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209487 in SH-SY5Y whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

• IHC-P

AbReview75513****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde fixed rat brain (locus coeruleus) tissue. Stained with ab209487 at 1/1000 dilution. Secondary antibody used was Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated at 1/200 dilution. Blocking was done with 1% BSA for 10 minutes at 21°C. The sample was incubated with the primary antibody in 1% BSA w/ 0.01% Azide for 16 hours at 21°C. Antigen retrieval method was heat mediated Citrate pH 6.4.

This image is courtesy of an Abreview submitted by Yazead Buhidma

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on chromaffin cells of rat adrenal gland medulla, and negative on adrenal cortex (PMID : 17535872, PMID : 176995660).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on chromaffin cells of mouse adrenal gland medulla, and negative on adrenal cortex (PMID : 17535872, PMID : 176995660).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative controls : HeLa (PMID : 7688735; PMID : 9763470, PMID : 15585414, PMID : 10037744), C6 (PMID : 9763470, PMID : 7798950, PMID : 10037744), HepG2 (PMID : 7798950, PMID : 11943777, PMID : 15585414).

All lanes:

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 75 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (AB209487)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 minutes; Lane 2 : 1 second.

All lanes:

Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487) at 1/1000 dilution

Lane 1:

Mouse adrenal gland lysate at 10 µg

Lane 2:

Rat adrenal gland lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 75 kDa

false