Rabbit Recombinant Monoclonal Dopamine beta Hydroxylase antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunoprecipitation-img11137.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74334.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img63332.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44296.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (AB223130)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Predicted | Expected | Predicted |
| Rat | Predicted | Expected | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Tris/EDTA Buffer, pH9 (ab93684) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.
Alternative names
Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, DBH
Recommended products
Rabbit Recombinant Monoclonal Dopamine beta Hydroxylase antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20385
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab223130 is the carrier-free version of Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunoprecipitation-img11137.jpg "Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)")
Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)
Dopamine beta Hydroxylase was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: SH-SY5Y whole cell lysate, 10 μg (Input).
Lane 2: Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487).
All lanes: Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487)
Predicted band size: 69 kDa
Observed band size: 75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74334.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Dopamine beta Hydroxylase with Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Strong cytoplasmic staining on chromaffin cells of human adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 17699566).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)
Immunohistochemical analysis of paraffin-embedded human adrenal pheochromocytoma tissue labeling Dopamine beta Hydroxylase with Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on tumor cells of human adrenal pheochromocytoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img63332.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Dopamine beta Hydroxylase with Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on chromaffin cells of mouse adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130), expandable thumbnail](https://content.abcam.com/products/images/dopamine-beta-hydroxylase-antibody-epr20385-bsa-and-azide-free-ab223130--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44296.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Dopamine beta Hydroxylase with Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on chromaffin cells of rat adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dopamine beta Hydroxylase antibody [EPR20385] ab209487).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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