Anti-Dopamine D2 Receptor antibody [EPR30297-57]

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (AB324734)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat striatum (fresh frozen) tissue labeling Dopamine D2 Receptor with ab324734 at 1/50 (9.86 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Panel A : merged staining of anti-Dopamine D2 Receptor (ab324734, green), anti-NeuN (ab190565, white) and anti-GFAP (ab201732, magenta) on rat striatum.
Panel B : anti-Dopamine D2 Receptor stained on rat striatum.
Panel C : anti-NeuN stained in neurons of rat striatum.
Panel D : anti-GFAP stained in astrocytes of rat striatum.
The section was incubated in two rounds of staining : in the order of ab324734 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (AB324734)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum (fresh frozen) tissue labeling Dopamine D2 Receptor with ab324734 at 1/50 (9.86 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Panel A : merged staining of anti-Dopamine D2 Receptor (ab324734, green), anti-NeuN (ab190565, white) and anti-GFAP (ab201732, magenta) on mouse striatum.
Panel B : anti-Dopamine D2 Receptor stained on mouse striatum.
Panel C : anti-NeuN stained in neurons of mouse striatum.
Panel D : anti-GFAP stained in astrocytes of mouse striatum.
The section was incubated in two rounds of staining : in the order of ab324734 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

• WB

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Western blot - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (AB324734)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : cerebellum(PMID : 7531978), kidney

The samples are unboiled.

The molecular weight observed is consistent with what have been described in literature (PMID : 28316027).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (ab324734) at 1/1000 dilution

Lane 1:

Mouse striatum tissue lysate at 40 µg

Lane 2:

Mouse cerebellum tissue lysate at 40 µg

Lane 3:

Mouse kidney tissue lysate at 40 µg

Lane 4:

Rat striatum tissue lysate at 40 µg

Lane 5:

Rat cerebellum tissue lysate at 40 µg

Lane 6:

Rat kidney tissue lysate at 40 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 70-100 kDa,36 kDa

false

Exposure time: 125s

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (AB324734)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh frozen) tissue labeling Dopamine D2 Receptor with ab324734 at 1/50 (9.86 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Low expression : no staining on rat cerebellum.
Panel A : merged staining of anti-Dopamine D2 Receptor (ab324734, green), anti-NeuN (ab190565, white) and anti-GFAP (ab201732, magenta) on rat cerebellum.
Panel B : anti-Dopamine D2 Receptor stained on rat cerebellum.
Panel C : anti-NeuN stained in neurons of rat cerebellum.
Panel D : anti-GFAP stained in astrocytes of rat cerebellum.
The section was incubated in two rounds of staining : in the order of ab324734 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (AB324734)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh frozen) tissue labeling Dopamine D2 Receptor with ab324734 at 1/50 (9.86 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Low expression : confocal image showing no staining on rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab324734 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (AB324734)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh frozen) tissue labeling Dopamine D2 Receptor with ab324734 at 1/50 (9.86 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Low expression : no staining on mouse cerebellum.
Panel A : merged staining of anti-Dopamine D2 Receptor (ab324734, green), anti-NeuN (ab190565, white) and anti-GFAP (ab201732, magenta) on mouse cerebellum.
Panel B : anti-Dopamine D2 Receptor stained on mouse cerebellum.
Panel C : anti-NeuN stained in neurons of mouse cerebellum.
Panel D : anti-GFAP stained in astrocytes of mouse cerebellum.
The section was incubated in two rounds of staining : in the order of ab324734 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dopamine D2 Receptor antibody [EPR30297-57] (AB324734)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh frozen) tissue labeling Dopamine D2 Receptor with ab324734 at 1/50 (9.86 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Low expression : confocal image showing no staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab324734 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.