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AB313857

Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free

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Rabbit Recombinant Monoclonal Dopamine D2 Receptor antibody. Carrier free. Suitable for FuncS, ICC/IF, WB and reacts with Synthetic peptide, Human, Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human DRD2.

View Alternative Names

D(2) dopamine receptor, Dopamine D2 receptor, DRD2, D(2) dopamine receptor, Dopamine D2 receptor, Drd2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)

Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells fixed in ice-cold MeOH for 5 minutes labeling Dopamine Receptor D2 with ab313857 at 1/1000 dilution (green). Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000. Blue : Fluoroshield with DAPI. Scale bar= 10um.

Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)

Immunofluorescent analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Dopamine Receptor D2 with ab313857 at 1/250 dilution (green). Red : Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1/500 dilution. Blue : Fluoroshield with DAPI.

Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)
  • WB

Supplier Data

Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (ab313857) at 1/1000 dilution

All lanes:

TF-1 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Functional Studies - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)
  • FuncS

Supplier Data

Functional Studies - Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (AB313857)

ab313857 specifically detects DRD2 protein on a VirDTM-GPCR array. Yellow : DRD2 White : DRD3 Blue : DRD5 Bottom left : Positive control

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1478

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, FuncS, WB

applications

Immunogen

Recombinant Fragment Protein within Human DRD2.

P14416

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "FuncS-species-checked": "testedAndGuaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase (PubMed : 21645528). Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5 (By similarity).
See full target information DRD2

Additional targets

Drd2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com