Rat Recombinant Monoclonal Dopamine Receptor D1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.
IgG2a
Rat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-Fr | IHC-P | WB | |
---|---|---|---|
Mouse | Not recommended | Tested | Tested |
Rat | Not recommended | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Drd1a, Gpcr15, Drd1, Drd1a, Gpcr15, D(1A) dopamine receptor, Dopamine D1 receptor
Rat Recombinant Monoclonal Dopamine Receptor D1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.
IgG2a
Rat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
1-1-F11s.E6
Ion exchange chromatography
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Lanes 1 - 2: Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free ab256556)
Lane 1: Mouse striatum lysate at 20 µg
Lane 2: Rat striatum lysate at 20 µg
All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Observed band size: 75-100 kDa
Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
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