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Rat Recombinant Monoclonal Dopamine Receptor D1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555), expandable thumbnail
  • Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (AB256555), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Rat

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrIHC-PWB
Mouse
Not recommended
Tested
Tested
Rat
Not recommended
Tested
Tested

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Target data

Function

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Alternative names

Recommended products

Rat Recombinant Monoclonal Dopamine Receptor D1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Clone number

1-1-F11s.E6

Purification technique

Ion exchange chromatography

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)

    Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)

    Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)

    Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555), expandable thumbnail

    Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)

    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    Lanes 1 - 2: Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free ab256556)

    Lane 1: Mouse striatum lysate at 20 µg

    Lane 2: Rat striatum lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Observed band size: 75-100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (ab256555)

    Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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