Rat Recombinant Monoclonal Dopamine Receptor D1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425350.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425352.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425351.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556)")
![Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--western-blot-img425348.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425349.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (AB256556)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-Fr | IHC-P | WB | |
|---|---|---|---|
| Mouse | Not recommended | Tested | Tested |
| Rat | Not recommended | Tested | Tested |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Alternative names
Drd1a, Gpcr15, Drd1, D(1A) dopamine receptor, Dopamine D1 receptor
Recommended products
Rat Recombinant Monoclonal Dopamine Receptor D1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- 1-1-F11s.E6
- Purification technique
- Ion exchange chromatography
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425350.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
This data was developed using Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425352.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
This data was developed using Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425351.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
This data was developed using Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)![Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--western-blot-img425348.jpg "Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)")
Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
This data was developed using Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBSTLanes 1 - 2: Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] (Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
Lane 1: Mouse striatum lysate at 20 µg
Lane 2: Rat striatum lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Observed band size: 75-100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556), expandable thumbnail](https://content.abcam.com/products/images/dopamine-receptor-d1-antibody-1-1-f11-se6-bsa-and-azide-free-ab256556--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425349.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] - BSA and Azide free (ab256556)
This data was developed using Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
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