Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)

Rabbit Recombinant Monoclonal Dopamine Receptor D1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	WB	IHC-Fr	IHC-P
Human	Not recommended	Not recommended	Expected	Tested	Tested
Mouse	Not recommended	Not recommended	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Tested	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Rat	Dilution info 1/50	Notes -
Species Human	Dilution info 1/50	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500 - 1/4000	Notes For rat and mouse samples recommended use at a 1/4000 dilution. For human samples recommended use at 1/500 dilution. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500 - 1/4000	Notes For rat and mouse samples recommended use at a 1/4000 dilution. For human samples recommended use at 1/500 dilution. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500 - 1/4000	Notes For rat and mouse samples recommended use at a 1/4000 dilution. For human samples recommended use at 1/500 dilution. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information DRD1

Function

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Alternative names

D(1A) dopamine receptor, Dopamine D1 receptor, DRD1

Recommended products

Rabbit Recombinant Monoclonal Dopamine Receptor D1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24102-105
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1-3: 15 seconds Lane 4-6: 69 seconds.
The molecular weight observed is consistent with what have been described in literature (PMID: 28316027).

Negative control: heart, kidney (PMID: 2144334)

Samples are non-boiled as boiling may cause protein aggregates.
All lanes: Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713) at 1/1000 dilution
Lane 1: Mouse striatum tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Rat striatum tissue lysate at 20 µg
Lane 5: Rat heart tissue lysate at 20 µg
Lane 6: Rat kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 95-100 kDa
Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
IHC image of Dopamine Receptor D1 staining in a section of frozen normal human thalamus* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining.
The section was incubated with ab279713, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of paraffin-embedded Human corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/500 (1.026 μg/ml) dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining is on the corpus striatum of human brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
IHC image of Dopamine Receptor D1 staining in a section of frozen normal human striatum* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining.
The section was incubated with ab279713, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen mouse striatum (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
Positive staining on the mouse striatum is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining is mainly on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen mouse kidney (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
No staining on the mouse kidney is observed.
Negative control: kidney (PMID: 2144334).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of paraffin-embedded Mouse corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of paraffin-embedded Rat corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen rat striatum (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
Positive staining on the rat striatum is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen rat kidney (fresh) tissue labeling Dopamine Receptor D1 with ab279713 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (green) at a 1/1000 dilution. The nuclear counterstain is DAPI (blue).
No staining on the rat kidney is observed.
Negative control: kidney (PMID: 2144334).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 dilution.