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AB130140

Anti-Dopamine Transporter antibody

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(1 Publication)

Goat Polyclonal Dopamine Transporter antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC6A3 aa 1-100.

View Alternative Names

DAT1, SLC6A3, Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3

1 Images
Western blot - Anti-Dopamine Transporter antibody (AB130140)
  • WB

Unknown

Western blot - Anti-Dopamine Transporter antibody (AB130140)

Primary incubation was 1 hour.

The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mazei-Robison et al, J Neurosci. 2008 Jul 9;28(28) : 7040-6. PMID : 18614672).

All lanes:

Western blot - Anti-Dopamine Transporter antibody (ab130140) at 1 µg/mL

All lanes:

Human Substantia nigra lysate (in RIPA buffer) at 35 µg

Predicted band size: 68 kDa

Observed band size: 85 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLC6A3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q01959

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab130140 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Mediates sodium- and chloride-dependent transport of dopamine (PubMed : 10375632, PubMed : 11093780, PubMed : 1406597, PubMed : 15505207, PubMed : 19478460, PubMed : 8302271). Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity).
See full target information SLC6A3

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 10:806-815 PubMed29636870

2018

Reverse mode of sodium/calcium exchanger subtype 1 contributes to detrusor overactivity in rats with partial bladder outflow obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhong,Nan You,Qingqing Wang,Longkun Li,Chibing Huang
View all publications

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