Rabbit Polyclonal Dopamine Transporter phospho T53 antibody. Suitable for WB and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Rat Slc6a3 phospho T53 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- International journal of molecular sciences 22:2021Effects of β-Phenylethylamine on Psychomotor, Rewarding, and Reinforcing Behaviors and Affective State: The Role of Dopamine D1 Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIn Soo Ryu,Oc-Hee Kim,Ji Sun Kim,Sumin Sohn,Eun Sang Choe,Ri-Na Lim,Tae Wan Kim,Joung-Wook Seo,Eun Young JangPubMed 34502393
- British journal of pharmacology 178:3869-38872021Methoxphenidine (MXP) induced abnormalities: Addictive and schizophrenia-related behaviours based on an imbalance of neurochemicals in the brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKwang-Hyun Hur,Seong-Eon Kim,Shi-Xun Ma,Bo-Ram Lee,Yong-Hyun Ko,Jee-Yeon Seo,Seon-Kyung Kim,Young-Jung Kim,Su-Jeong Sung,Youyoung Lee,Young Hoon Jung,Yong-Sup Lee,Seok-Yong Lee,Choon-Gon JangPubMed 33987827
- International journal of molecular sciences 22:2021Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUlrika H Julku,Maria Jäntti,Reinis Svarcbahs,Timo T MyöhänenPubMed 33579026
- Molecular neurobiology 55:470-4822016Prolyl Oligopeptidase Regulates Dopamine Transporter Phosphorylation in the Nigrostriatal Pathway of Mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUlrika H Julku,Anne E Panhelainen,Saija E Tiilikainen,Reinis Svarcbahs,Anne E Tammimäki,T Petteri Piepponen,Mari H Savolainen,Timo T MyöhänenPubMed 27966077
- The Journal of biological chemistry 287:29702-122012Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames D Foster et. al.PubMed 22722938
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Slc6a3 phospho T53 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23977
Reactivity data
Select an application| WB | |
|---|---|
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Mediates sodium- and chloride-dependent transport of dopamine (PubMed:1502198, PubMed:1765147, PubMed:1948034, PubMed:1948035, PubMed:8125921). Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (PubMed:8125921). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity).
Alternative names
Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3, Slc6a3
Recommended products
Rabbit Polyclonal Dopamine Transporter phospho T53 antibody. Suitable for WB and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Rat Slc6a3 phospho T53 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Slc6a3 phospho T53 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23977
- Purification technique
- Affinity purification Immunogen
- Specificity
ab183486 is specific for the ~55kDa glycosylated form of the Dopamine Transporter protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the Dopamine Transporter protein. Immunolabeling of the Dopamine Transporter band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
- Concentration
- Purification notes
ab183486 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Dopamine Transporter (phospho T53) antibody (ab183486)
All lanes: Western blot - Anti-Dopamine Transporter (phospho T53) antibody (ab183486) at 1/1000 dilution
Lane 1: Rat caudate lysate
Lane 2: Rat caudate lysate with immunizing phosphopeptide
Predicted band size: 68 kDa
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