AB183486

Anti-Dopamine Transporter (phospho T53) antibody

Name: Anti-Dopamine Transporter (phospho T53) antibody (ab183486) | Abcam
Brand: Abcam Limited
SKU: AB183486
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Dopamine Transporter phospho T53 antibody. Suitable for WB and reacts with Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Rat Slc6a3 pT53 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3, Slc6a3

1 Images

Supplier Data

Western blot - Anti-Dopamine Transporter (phospho T53) antibody (AB183486)

All lanes:

Western blot - Anti-Dopamine Transporter (phospho T53) antibody (ab183486) at 1/1000 dilution

Lane 1:

Rat caudate lysate

Lane 2:

Rat caudate lysate with immunizing phosphopeptide

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Rat Slc6a3 pT53 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P23977

Specificity

ab183486 is specific for the ~55kDa glycosylated form of the Dopamine Transporter protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the Dopamine Transporter protein. Immunolabeling of the Dopamine Transporter band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab183486 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates sodium- and chloride-dependent transport of dopamine (PubMed : 1502198, PubMed : 1765147, PubMed : 1948034, PubMed : 1948035, PubMed : 8125921). Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (PubMed : 8125921). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity).

See full target information Slc6a3 pT53

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry 29:3607-3622 PubMed38822069

2024

The ion channel TRPA1 is a modulator of the cocaine reward circuit in the nucleus accumbens.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Jung Kim,Su Jeong Choi,Sa-Ik Hong,Jung-Cheol Park,Youyoung Lee,Shi-Xun Ma,Kwang-Hyun Hur,Young Lee,Kyeong-Man Kim,Hyung Kyu Kim,Hee Young Kim,Seok-Yong Lee,Se-Young Choi,Choon-Gon Jang

CNS neuroscience & therapeutics 29:2608-2620 PubMed37002793

2023

Electroacupuncture alleviated depression-like behaviors in ventromedial prefrontal cortex of chronic unpredictable mild stress-induced rats: Increasing synaptic transmission and phosphorylating dopamine transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Cai,Mei Wu,Zhinan Zhang,Huacong Liu,Shengtao Huang,Jia Song,Siqiang Ren,Yong Huang

International journal of molecular sciences 22: PubMed34502393

2021

Effects of β-Phenylethylamine on Psychomotor, Rewarding, and Reinforcing Behaviors and Affective State: The Role of Dopamine D1 Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

In Soo Ryu,Oc-Hee Kim,Ji Sun Kim,Sumin Sohn,Eun Sang Choe,Ri-Na Lim,Tae Wan Kim,Joung-Wook Seo,Eun Young Jang

British journal of pharmacology 178:3869-3887 PubMed33987827

2021

Methoxphenidine (MXP) induced abnormalities: Addictive and schizophrenia-related behaviours based on an imbalance of neurochemicals in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Kwang-Hyun Hur,Seong-Eon Kim,Shi-Xun Ma,Bo-Ram Lee,Yong-Hyun Ko,Jee-Yeon Seo,Seon-Kyung Kim,Young-Jung Kim,Su-Jeong Sung,Youyoung Lee,Young Hoon Jung,Yong-Sup Lee,Seok-Yong Lee,Choon-Gon Jang

International journal of molecular sciences 22: PubMed33579026

2021

Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrika H Julku,Maria Jäntti,Reinis Svarcbahs,Timo T Myöhänen

Molecular neurobiology 55:470-482 PubMed27966077

2016

Prolyl Oligopeptidase Regulates Dopamine Transporter Phosphorylation in the Nigrostriatal Pathway of Mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrika H Julku,Anne E Panhelainen,Saija E Tiilikainen,Reinis Svarcbahs,Anne E Tammimäki,T Petteri Piepponen,Mari H Savolainen,Timo T Myöhänen

The Journal of biological chemistry 287:29702-12 PubMed22722938

2012

Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux.

Applications

Unspecified application

Species

Unspecified reactive species

James D Foster,Jae-Won Yang,Amy E Moritz,Sathyavathi Challasivakanaka,Margaret A Smith,Marion Holy,Kyle Wilebski,Harald H Sitte,Roxanne A Vaughan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dopamine Transporter (phospho T53) antibody

Western blot - Anti-Dopamine Transporter (phospho T53) antibody (AB183486)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise