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AB251750

Anti-DORFIN antibody

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(2 Publications)

Rabbit Polyclonal DORFIN antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RNF19A aa 100 to C-terminus.

View Alternative Names

RNF19, RNF19A, E3 ubiquitin-protein ligase RNF19A, Double ring-finger protein, RING finger protein 19A, p38, Dorfin

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DORFIN antibody (AB251750)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DORFIN antibody (AB251750)

Paraffin-embedded human prostate tissue stained for DORFIN using ab251750 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DORFIN antibody (AB251750)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DORFIN antibody (AB251750)

Paraffin-embedded human testis tissue stained for DORFIN using ab251750 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-DORFIN antibody (AB251750)
  • WB

Unknown

Western blot - Anti-DORFIN antibody (AB251750)

All lanes:

Western blot - Anti-DORFIN antibody (ab251750) at 0.4 µg

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RNF19A aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NV58

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DORFIN also known as RNF19A is an E3 ubiquitin-protein ligase with significant roles in cellular protein degradation. It has a molecular mass of approximately 46 kDa. DORFIN is expressed in several tissues with higher expression noted in the brain and testis. It plays a critical role in the ubiquitin-proteasome system facilitating the tagging of proteins for degradation.
Biological function summary

DORFIN functions by promoting the degradation of misfolded or damaged proteins. It is a component of protein quality control machinery ensuring cellular homeostasis. DORFIN often works in concert with other ubiquitin ligases and proteins including the C-terminus of Hsc70-interacting protein (CHIP) to maintain protein stability. This synergistic action supports cellular stress response and protects against proteotoxic stress.

Pathways

DORFIN participates in the protein ubiquitination and proteasome-mediated degradation pathway. It interacts with and possibly modulates key proteins in the apoptosis pathway such as Parkin. These interactions suggest a role in regulating cell survival and death important for maintaining cellular balance during stress conditions.

DORFIN's dysregulation has been associated with neurodegenerative diseases notably Parkinson's disease. The relationship with proteins like Parkin connects DORFIN to the pathology seen in these disorders suggesting its importance in maintaining neuronal integrity. Additionally abnormalities in DORFIN activity are linked to certain types of cancer as it may influence the stability and degradation of oncogenic proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.
See full target information RNF19A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pathogens (Basel, Switzerland) 12: PubMed37375542

2023

TRIM16 Overexpression in HEK293T Cells Results in Cell Line-Specific Antiviral Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Lance R Nigos,Nichollas E Scott,Andrew G Brooks,Malika Ait-Goughoulte,Sarah L Londrigan,Patrick C Reading,Rubaiyea Farrukee

Nature communications 12:6653 PubMed34789768

2021

RNF19A-mediated ubiquitination of BARD1 prevents BRCA1/BARD1-dependent homologous recombination.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhu,Jinzhou Huang,Hongyang Huang,Huan Li,Peiqiang Yi,Jake A Kloeber,Jian Yuan,Yuping Chen,Min Deng,Kuntian Luo,Ming Gao,Guijie Guo,Xinyi Tu,Ping Yin,Yong Zhang,Jun Su,Jiayi Chen,Zhenkun Lou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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