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Mouse Monoclonal Doublecortin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DCX.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (AB254133), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (AB254133), expandable thumbnail
  • Western blot - Anti-Doublecortin antibody [3E1] (AB254133), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

Preservative: 0.065% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human DCX. Database link O43602

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Rat
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

Alternative names

DBCN, LISX, DCX, Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X

Recommended products

Mouse Monoclonal Doublecortin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DCX.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human DCX. Database link O43602
Clone number
3E1
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (ab254133), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (ab254133)

    Immunofluorescent analysis of rat cortical neuron-glial cells using ab254133 at 1/1000 dilution ab254133 (red). An anti-MAP2 (1/10,000, green) labels dendrites and perikarya or mature neurons. The nuclear counter stain is DAPI (blue). Doublecortin is an excellent marker of early developing neuronal cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (ab254133), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (ab254133)

    Immunofluorescent analysis of cultured rat neurons showing strong cytoplasmic staining of Doublecortin using ab254133 at 1/1000 dilution (green) in developing neurons. GFAP is shown in red.

  • Western blot - Anti-Doublecortin antibody [3E1] (ab254133), expandable thumbnail

    Western blot - Anti-Doublecortin antibody [3E1] (ab254133)

    All lanes: Western blot - Anti-Doublecortin antibody [3E1] (ab254133) at 1/5000 dilution

    All lanes: 3 day post natal rat brain lysate

    Predicted band size: 49 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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