AB254133

Anti-Doublecortin antibody [3E1]

Name: Anti-Doublecortin antibody [3E1] (ab254133) | Abcam
Brand: Abcam Limited
SKU: AB254133

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(6 Publications)

Mouse Monoclonal Doublecortin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DCX.

View Alternative Names

DBCN, LISX, DCX, Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (AB254133)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (AB254133)

Immunofluorescent analysis of rat cortical neuron-glial cells using ab254133 at 1/1000 dilution ab254133 (red). An anti-MAP2 (1/10,000, green) labels dendrites and perikarya or mature neurons. The nuclear counter stain is DAPI (blue). Doublecortin is an excellent marker of early developing neuronal cells.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [3E1] (AB254133)

Immunofluorescent analysis of cultured rat neurons showing strong cytoplasmic staining of Doublecortin using ab254133 at 1/1000 dilution (green) in developing neurons. GFAP is shown in red.

Supplier Data

Western blot - Anti-Doublecortin antibody [3E1] (AB254133)

All lanes:

Western blot - Anti-Doublecortin antibody [3E1] (ab254133) at 1/5000 dilution

All lanes:

3 day post natal rat brain lysate

Predicted band size: 49 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E1

Isotype

IgG2a

Carrier free

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DCX.

O43602

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Alternative Products

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Primary Antibodies

AB222921

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Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.065% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

See full target information DCX

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9529 PubMed39532843

2024

Nuclear localization of MTHFD2 is required for correct mitosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Pardo-Lorente,Anestis Gkanogiannis,Luca Cozzuto,Antoni Gañez Zapater,Lorena Espinar,Ritobrata Ghose,Jacqueline Severino,Laura García-López,Rabia Gül Aydin,Laura Martin,Maria Victoria Neguembor,Evangelia Darai,Maria Pia Cosma,Laura Batlle-Morera,Julia Ponomarenko,Sara Sdelci

Journal of neurochemistry 164:624-642 PubMed36453259

2022

Involvement of brain-derived neurotrophic factor methylation in the prefrontal cortex and hippocampus induced by chronic unpredictable mild stress in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyue Cheng,Wei Wang,Zemeng Zhu,Mingyue Zhao,Hannao Li,Dexiang Liu,Fang Pan

CNS neuroscience & therapeutics 28:1081-1092 PubMed35481944

2022

Downregulation of microRNA-124-3p promotes subventricular zone neural stem cell activation by enhancing the function of BDNF downstream pathways after traumatic brain injury in adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

En-Ming Kang,Yi-Bin Jia,Jia-You Wang,Guan-Yi Wang,Hui-Jun Chen,Xiao-Yan Chen,Yu-Qin Ye,Xin Zhang,Xin-Hong Su,Jing-Yu Wang,Xiao-Sheng He

Journal of neuroinflammation 18:254 PubMed34736493

2021

Rifaximin-mediated gut microbiota regulation modulates the function of microglia and protects against CUMS-induced depression-like behaviors in adolescent rat.

Applications

Unspecified application

Species

Unspecified reactive species

Haonan Li,Yujiao Xiang,Zemeng Zhu,Wei Wang,Zhijun Jiang,Mingyue Zhao,Shuyue Cheng,Fang Pan,Dexiang Liu,Roger C M Ho,Cyrus S H Ho

Molecular biology of the cell 33:ar52 PubMed34705476

2021

Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.

Applications

Unspecified application

Species

Unspecified reactive species

Erin M Masucci,Peter K Relich,Melike Lakadamyali,E Michael Ostap,Erika L F Holzbaur

Journal of pain research 14:1061-1070 PubMed33907454

2021

Curcumin Alleviates Chronic Pain and Improves Cognitive Impairment via Enhancing Hippocampal Neurogenesis in Sciatic Nerve Constriction Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Du,Yifan Deng,Zhuolin Qiu,Guoliang Sun,Yue Guo,Ziqing Hei,Xiang Li

View all publications

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Product promise

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