Rabbit Recombinant Monoclonal Doublecortin antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human DCX aa 200-250.
Images
![Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--western-blot-img420532.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)")
![Immunoprecipitation - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunoprecipitation-img420533.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420534.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420535.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)")
![Immunocytochemistry - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunocytochemistry-img420536.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human DCX aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43602
Reactivity data
Select an application| ICC | IHC-P | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Alternative names
DBCN, LISX, DCX, Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X
Recommended products
Rabbit Recombinant Monoclonal Doublecortin antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human DCX aa 200-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human DCX aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43602
- Clone number
- BLR168J
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--western-blot-img420532.jpg "Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)")
Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)
Samples: Whole cell lysate (50 µg) from HEK293T, SK-NBE(2), HeLa, SK-N-SH, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168) used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 30 seconds.
All lanes: Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076) at 1/1000 dilution
Lane 1: HEK293T Whole cell lysate at 50 µg
Lane 2: SK-NBE(2) Whole cell lysate at 50 µg
Lane 3: HeLa Whole cell lysate at 50 µg
Lane 4: SK-N-SH Whole cell lysate at 50 µg
Lane 5: Hep-G2 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat antirabbit IgG
![Immunoprecipitation - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunoprecipitation-img420533.jpg "Immunoprecipitation - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)")
Immunoprecipitation - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168-1) used for IP at 20 µl/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin (BL-1974A-2B1). For blotting immunoprecipitated Doublecortin, A700-168 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420534.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)
Detection of human Doublecortin in FFPE astrocytoma by immunohistochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168). Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420535.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)
Detection of mouse Doublecortin in FFPE juvenile mouse brain (dentate gyrus) by immunohistochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunocytochemistry - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-blr168j-bsa-free-ab314076--immunocytochemistry-img420536.jpg "Immunocytochemistry - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)")
Immunocytochemistry - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076)
Detection of human Doublecortin in FFPE SK-N-BE(2) by immunocytochemistry. Antibody: Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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