AB314076

Anti-Doublecortin antibody [BLR168J] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal Doublecortin antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human DCX aa 200-250.

View Alternative Names

DBCN, LISX, DCX, Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Detection of human Doublecortin in FFPE astrocytoma by immunohistochemistry. Antibody : Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168). Secondary : HRP-conjugated goat antirabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Detection of human Doublecortin in FFPE SK-N-BE(2) by immunocytochemistry. Antibody : Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168-1) used for IP at 20 µl/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin (BL-1974A-2B1). For blotting immunoprecipitated Doublecortin, A700-168 was used at 1 : 1000. Chemiluminescence with an exposure time of 10 seconds.

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Detection of mouse Doublecortin in FFPE juvenile mouse brain (dentate gyrus) by immunohistochemistry. Antibody : Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Samples : Whole cell lysate (50 µg) from HEK293T, SK-NBE(2), HeLa, SK-N-SH, and Hep-G2 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Doublecortin recombinant monoclonal antibody [BLR168J] (A700-168) used at 1 : 1000. Secondary : HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (ab314076) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 50 µg

Lane 2:

SK-NBE(2) Whole cell lysate at 50 µg

Lane 3:

HeLa Whole cell lysate at 50 µg

Lane 4:

SK-N-SH Whole cell lysate at 50 µg

Lane 5:

Hep-G2 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat antirabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR168J

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P, IP, ICC

applications

Immunogen

Synthetic Peptide within Human DCX aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

O43602

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

See full target information DCX

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Doublecortin antibody [BLR168J] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Immunocytochemistry - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Immunoprecipitation - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Western blot - Anti-Doublecortin antibody [BLR168J] - BSA free (AB314076)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise