Rabbit Recombinant Monoclonal Doublecortin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Images
![Western blot - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (AB249418), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-epr10935b-bsa-and-azide-free-ab249418--western-blot-img57437.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (AB249418)")
![Flow Cytometry (Intracellular) - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (AB249418), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-epr10935b-bsa-and-azide-free-ab249418--flow-cytometry-intracellular-img33209.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (AB249418)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Expected |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Not recommended | Expected | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Alternative names
DBCN, LISX, DCX, Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X
Recommended products
Rabbit Recombinant Monoclonal Doublecortin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10935(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249418 is the carrier-free version of Anti-Doublecortin antibody [EPR10935(B)] ab167400.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (ab249418), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-epr10935b-bsa-and-azide-free-ab249418--western-blot-img57437.jpg "Western blot - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (ab249418)")
Western blot - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (ab249418)
This data was developed using Anti-Doublecortin antibody [EPR10935(B)] ab167400, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Doublecortin antibody [EPR10935(B)] (Anti-Doublecortin antibody [EPR10935(B)] ab167400) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: C6 lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa
![Flow Cytometry (Intracellular) - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (ab249418), expandable thumbnail](https://content.abcam.com/products/images/doublecortin-antibody-epr10935b-bsa-and-azide-free-ab249418--flow-cytometry-intracellular-img33209.jpg "Flow Cytometry (Intracellular) - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (ab249418)")
Flow Cytometry (Intracellular) - Anti-Doublecortin antibody [EPR10935(B)] - BSA and Azide free (ab249418)
This data was developed using Anti-Doublecortin antibody [EPR10935(B)] ab167400, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized C6 cells labeling Doublecortin with Anti-Doublecortin antibody [EPR10935(B)] ab167400 at 1/100 dilution (red) or a Rabbit IgG (negative) (green).
Downloads
Product protocols
- Visit the general protocols
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