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Anti-Doublecortin antibody [EPR19997] (ab207175) is a rabbit monoclonal antibody detecting Doublecortin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications



Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (AB207175), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (AB207175), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (AB207175), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Doublecortin antibody [EPR19997] (AB207175), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [EPR19997] (AB207175), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-FrIHC-P
Human
Tested
Tested
Not recommended
Expected
Mouse
Tested
Expected
Not recommended
Tested
Rat
Tested
Expected
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). IHC-Fr is only recommended for rat species.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). IHC-Fr is only recommended for rat species.

Species
Mouse
Dilution info
-
Notes

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). IHC-Fr is only recommended for rat species.

Tested
Tested

Species
Mouse
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

Alternative names

DBCN, LISX, DCX, Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X

Recommended products

Anti-Doublecortin antibody [EPR19997] (ab207175) is a rabbit monoclonal antibody detecting Doublecortin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19997
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Doublecortin antibody [EPR19997] (ab207175) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Doublecortin?


Anti-Doublecortin [EPR19997] (ab207175) specifically detects a band for Doublecortin (UniProt: O43602) at a molecular weight of 40kDa.

Trusted by the scientific community


Anti-Doublecortin [EPR19997] (ab207175) was first used in a scientific publication in 2016 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR19997] also available for your convenience:
ab207175, Carrier free - Anti-Doublecortin antibody [EPR19997] - BSA and Azide free ab222921, Carrier free - Anti-Doublecortin antibody [EPR19997] - BSA and Azide free (Detector) ab244906, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Doublecortin antibody [EPR19997] ab305266, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Doublecortin antibody [EPR19997] ab307383, Biotin - Biotin Anti-Doublecortin antibody [EPR19997] (Detector) ab322840



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)

    IHC image of Doublecortin staining in a formalin fixed, paraffin embedded normal rat hippocampus (SVZ region) tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab207175 at 1/500 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. As a negative control (inset), an identical assay was performed without adding the primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)

    Immunohistochemical analysis of paraffin-embedded mouse adult hippocampus tissue labeling Doublecortin with ab207175 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on mouse hippocampal dentate gyrus is observed [PMID:23690918] [PMID:16814555]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)

    Immunohistochemical analysis of paraffin-embedded rat adult hippocampus tissue labeling Doublecortin with ab207175 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on rat hippocampal dentate gyrus is observed [PMID:23690918] [PMID:16814555]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat adult hippocampus tissue labeling Doublecortin with ab207175 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on rat hippocampal dentate gyrus is observed [PMID:23690918] [PMID:16814555]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [EPR19997] (ab207175)

    Immunofluorescent analysis of 100% Methanol-fixed SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Doublecortin with ab207175 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The higher molecular weight band is phosphorylated form and the lower molecular weight band is non-phosphorylated form. (PMID: 17178868).

    Doublecortin is highly expressed during embryonic development and downregulated in adult tissues. (PMID: 10399933).

    All lanes: Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175) at 1/10000 dilution

    Lane 1: Human fetal brain lysate at 20 µg

    Lane 2: Human brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 40 kDa

    Exposure time: 3s

  • Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lane 2: 3 minutes.

    The higher molecular weight band is phosphorylated form and the lower molecular weight band is non-phosphorylated form. (PMID: 17178868).

    Doublecortin is highly expressed during embryonic development and downregulated in adult tissues. (PMID: 10399933).

    All lanes: Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175) at 1/10000 dilution

    Lane 1: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

    Lane 2: Mouse brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175), expandable thumbnail

    Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-4: 3 minutes; Lane 5/6: 10 seconds.

    The observed expression profile is consistent with what has been described in the literature (PMID: 10550327).

    All lanes: Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Mouse brain lysate at 10 µg

    Lane 4: Mouse heart lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: Rat heart lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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