AB124678

Anti-DP1 antibody [EPR4959(2)]

Name: Anti-DP1 antibody [EPR4959(2)] (ab124678) | Abcam
Brand: Abcam Limited
SKU: AB124678
Price: 190 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal DP1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

DP1, TFDP1, Transcription factor Dp-1, DRTF1-polypeptide 1, E2F dimerization partner 1, DRTF1

2 Images

Flow Cytometry (Intracellular) - Anti-DP1 antibody [EPR4959(2)] (AB124678)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DP1 antibody [EPR4959(2)] (AB124678)

ab124678 at 1/10 dilution staining DP1 in permeabilized HeLa cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).

Unknown

Western blot - Anti-DP1 antibody [EPR4959(2)] (AB124678)

All lanes:

Western blot - Anti-DP1 antibody [EPR4959(2)] (ab124678) at 1/10000 dilution

All lanes:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 45 kDa

Observed band size: 49 kDa

false

Carrier free

Anti-DP1 antibody [EPR4959(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4959(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DP1 receptor also known as the prostaglandin D2 receptor 1 is a G-protein-coupled receptor with a molecular mass of approximately 44 kDa. This receptor binds to prostaglandin D2 (PGD2) and is found in various tissues including the central nervous system lungs and some immune cells. The presence of DP1 in these tissues suggests a role in multiple physiological processes reflecting its distribution in the body.

Biological function summary

Prostaglandin D2 (PGD2) plays a significant role in mediating inflammatory and allergic responses. The DP1 receptor is part of a signaling complex that modulates smooth muscle relaxation inhibition of platelet aggregation and sleep induction. Its expression in both neural and peripheral tissues underlines its involvement in a range of biological functions including the regulation of sleep-wake cycles and inflammation.

Pathways

The DP1 receptor contributes significantly to the prostaglandin signaling pathway which involves other prostaglandin receptors such as DP2 and EP receptors. This pathway mediates various physiological responses including vasodilation and immune modulation. The signaling interactions between DP1 and these related receptors help coordinate complex biological processes such as the regulation of vascular tone and inflammatory responses.

Abnormal DP1 signaling relates to asthma and allergic rhinitis conditions where inflammation and immune response are dysregulated. Overactivation or altered regulation of DP1 can exacerbate these diseases often involving other proteins like COX-2 which elevate levels of PGD2 during inflammation. Understanding DP1's role offers potential therapeutic angles for managing allergic and inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed : 7739537, PubMed : 8405995). The E2F1 : DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed : 20176812).

See full target information TFDP1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

mSphere 8:e0005623 PubMed36883841

2023

The E2F4/p130 Repressor Complex Cooperates with Oncogenic ΔNp73α To Inhibit Gene Expression in Human Papillomavirus 38 E6/E7-Transformed Keratinocytes and in Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valerio Taverniti,Hanna Krynska,Assunta Venuti,Marie-Laure Straub,Cécilia Sirand,Eugenie Lohmann,Maria Carmen Romero-Medina,Stefano Moro,Alexis Robitaille,Luc Negroni,Denise Martinez-Zapien,Murielle Masson,Massimo Tommasino,Katia Zanier

Annals of translational medicine 10:274 PubMed35433966

2022

GeneChip expression profiling identified as a potential therapeutic target in TNBC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufei Gao,Zimei Yang,Chuchu Xu,Qinghong Yu,Mengqian Wang,Jiaqing Song,Chunyu Wu,Mingcang Chen

The Journal of biological chemistry 298:101847 PubMed35314195

2022

Capsaicin inhibits intestinal Cl secretion and promotes Na absorption by blocking TRPV4 channels in healthy and colitic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxing Wan,Xiong Ying Chen,Fenglian Zhang,Jun Chen,Fenglan Chu,Zachary M Sellers,Feng Xu,Hui Dong

eLife 11: PubMed35212626

2022

KDM6B interacts with TFDP1 to activate P53 signaling in regulating mouse palatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tingwei Guo,Xia Han,Jinzhi He,Jifan Feng,Junjun Jing,Eva Janečková,Jie Lei,Thach-Vu Ho,Jian Xu,Yang Chai

Journal of experimental & clinical cancer research 39:216 PubMed33054826

2020

Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Huakai Wang,Shiyong Yu,Huan Peng,Yijun Shu,Wenjie Zhang,Qi Zhu,Yingxia Wu,Yijun Xu,Jiqi Yan,Honggang Xiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DP1 antibody [EPR4959(2)]

Flow Cytometry (Intracellular) - Anti-DP1 antibody [EPR4959(2)] (AB124678)

Western blot - Anti-DP1 antibody [EPR4959(2)] (AB124678)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise