AB235830

Anti-DP2 antibody

Name: Anti-DP2 antibody (ab235830) | Abcam
Brand: Abcam Limited
SKU: AB235830

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(3 Publications)

Rabbit Polyclonal DP2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TFDP2 aa 300-400.

View Alternative Names

DP2, TFDP2, Transcription factor Dp-2, E2F dimerization partner 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP2 antibody (AB235830)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP2 antibody (AB235830)

Paraffin-embedded human gastric cancer tissue stained for DP2 using ab235830 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP2 antibody (AB235830)

Paraffin-embedded human kidney tissue stained for DP2 using ab235830 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DP2 antibody (AB235830)

All lanes:

Western blot - Anti-DP2 antibody (ab235830) at 1/1000 dilution

Lane 1:

Mouse heart lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TFDP2 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q14188

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DP2 also known as CRTH2 or GPR44 is a receptor from the class of G protein-coupled receptors (GPCRs). It is involved in the signaling pathways mediated by prostaglandins making it an essential mediator in inflammatory processes. The molecular mass of this receptor is approximately 44 kDa. DP2 is broadly expressed in various tissues including the lungs eosinophils Th2 cells and basophils highlighting its role in immune response and respiratory function. Its expression in numerous cell types underlines its versatile roles within the immune system.

Biological function summary

DP2 functions as a receptor for prostaglandin D2 (PGD2) an important lipid mediator in inflammation. DP2's activities are a part of PGD2-driven processes including the recruitment and activation of Th2 cells eosinophils and basophils which are integral to allergic responses. DP2 does not form part of a larger protein complex but operates in concert with prostanoids such as the D-type prostanoid (DP1) receptors highlighting its specific signaling pathway within the immune context. By mediating chemotaxis and activation of specific immune cells DP2 supports the perpetuation of Th2-type inflammation.

Pathways

DP2 plays a role in the prostaglandin signaling pathway and immune response network. It operates parallel to receptors like DP1 albeit delivering contrasting cellular outcomes. While DP1 typically signals via a cAMP-dependent pathway DP2 mediates its effects through calcium mobilization and beta-arrestin-dependent pathways. These pathways contribute to distinct yet overlapping roles in processes of allergy and inflammation illustrating how DP2 function is intricately connected with other signaling molecules including chemoattractant receptors involved in leukocyte trafficking.

The dysregulation of DP2 is associated with asthma and allergic rhinitis. Within the context of asthma DP2 expression correlates with severity and exacerbation of the disease. The receptor's role in mediating eosinophil and Th2 cell attraction makes it a target of interest for therapeutic interventions. In allergic rhinitis similar mechanisms involving DP2-mediated immune cell recruitment exacerbate mucosal inflammation during allergen exposure. DP2's interaction with other inflammatory mediators such as the cysteinyl leukotriene receptor family solidifies its position in the cascade of allergic and respiratory disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The TFDP2 : E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1 : DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed : 20176812).

See full target information TFDP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational vision science & technology 12:5 PubMed37133840

2023

Attenuation of Laser-Induced Choroidal Neovascularization by Blockade of Prostaglandin D2 Receptor 2.

Applications

Unspecified application

Species

Unspecified reactive species

Hirotsugu Soga,Tatsuya Inoue,Yoshihiro Urade,Takashi Ueta,Hidetoshi Kawashima,Toshikatsu Kaburaki,Makoto Aihara

Autophagy 19:1745-1763 PubMed36449354

2022

The effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple.

Applications

Unspecified application

Species

Unspecified reactive species

Runmin Che,Changhai Liu,Qi Wang,Wenyan Tu,Ping Wang,Chao Li,Xiaoqing Gong,Ke Mao,Hao Feng,Lili Huang,Pengmin Li,Fengwang Ma

Frontiers in immunology 11:623693 PubMed33664736

2021

Bone Marrow Mesenchymal Stem Cells Ameliorate Cisplatin-Induced Renal Fibrosis miR-146a-5p/Tfdp2 Axis in Renal Tubular Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wu,Chao Rong,Qing Zhou,Xin Zhao,Xue-Mei Zhuansun,Shan Wan,Mao-Min Sun,Shou-Li Wang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP2 antibody (AB235830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DP2 antibody (AB235830)

Western blot - Anti-DP2 antibody (AB235830)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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