Anti-DPD antibody [7D4]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DPD antibody [7D4] (AB54797)

ab54797 at 10 ug/ml staining DPD in human Hela cells by Immunocytochemistry / Immunofluorescence.

This image was generated using the ascites version of the product.

• Flow Cyt

Unknown

Flow Cytometry - Anti-DPD antibody [7D4] (AB54797)

Overlay histogram showing HepG2 cells stained with ab54797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54797, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

• WB

Unknown

Western blot - Anti-DPD antibody [7D4] (AB54797)

DPD antibody (ab54797) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-DPD antibody [7D4] (ab54797)

Predicted band size: 111 kDa

false