AB312868

Anti-DPD antibody [EPR26638-154] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal DPD antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Dihydropyrimidine dehydrogenase [NADP(+)], DHPDHase, DPD, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPYD

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Sandwich ELISA - Anti-DPD antibody [EPR26638-154] - BSA and Azide free (Detector) (AB312868)

sELISA

Supplier Data

Sandwich ELISA - Anti-DPD antibody [EPR26638-154] - BSA and Azide free (Detector) (AB312868)

Sandwich ELISA of ab312866 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26638-154

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPD also known as dihydropyrimidine dehydrogenase is an enzyme important for the breakdown of pyrimidines particularly uracil and thymine. The DPD protein has an important role in the initial step of pyrimidine catabolism. It catalyzes the NADPH-dependent reduction of these pyrimidines to dihydrouracil and dihydrothymine. It consists of 1025 amino acids giving it a mass of approximately 111 kDa. DPD is expressed in various tissues with significant expression in the liver and to a lesser extent in peripheral blood lymphocytes.

Biological function summary

Dihydropyrimidine dehydrogenase impacts nucleotide metabolism which is significant for cellular replication and repair processes. DPD exists as part of a multi-enzyme complex that handles pyrimidine metabolism. It serves an important regulatory function by controlling the level of pyrimidines within cells. This regulation keeps the balance of free nucleotides which is critical for DNA synthesis and overall cellular homeostasis.

Pathways

DPD holds an important position in pyrimidine metabolism pathways and the drug metabolism pathway specifically influencing fluoropyrimidine-based chemotherapeutic agents. Within the pyrimidine metabolic pathway DPD links to other enzymes that further breakdown pyrimidines into more basic compounds. In drug metabolism DPD plays a vital role by metabolizing drugs like 5-fluorouracil. Its activity affects how individuals process these medications linking it to enzymes like thymidylate synthase that share pathways in cancer treatment drug action.

DPD deficiency relates to a genetic disorder that causes an excessive build-up of uracil and thymine leading to developmental disorders and neurological problems. Additionally abnormalities in DPD activity influence the efficacy and toxicity of fluorouracil chemotherapy in cancer treatments. DPD enzymatic activity when not properly regulated can lead to severe toxic reactions especially in relation to therapies reliant on enzymes such as thymidylate synthase emphasizing the critical interplay between these proteins in the management of fluoropyrimidine drug treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pyrimidine base degradation (PubMed : 1512248). Catalyzes the reduction of uracil and thymine (PubMed : 1512248). Also involved the degradation of the chemotherapeutic drug 5-fluorouracil (PubMed : 1512248).

See full target information Dihydropyrimidine dehydrogenase [NADP(+)]

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DPD antibody [EPR26638-154] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-DPD antibody [EPR26638-154] - BSA and Azide free (Detector) (AB312868)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise