AB121380

Anti-DPEP1/MDP antibody

Name: Anti-DPEP1/MDP antibody (ab121380) | Abcam
Brand: Abcam Limited
SKU: AB121380

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(1 Publication)

Rabbit Polyclonal DPEP1/MDP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DPEP1 aa 100-250.

View Alternative Names

MDP, RDP, DPEP1, Dipeptidase 1, Beta-lactamase, Dehydropeptidase-I, Microsomal dipeptidase, Renal dipeptidase, hRDP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DPEP1/MDP antibody (AB121380)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DPEP1/MDP antibody (AB121380)

Immunocytochemistry/immunofluorescence analysis of HepG2 cells labelling DPEP1/MDP with ab121380 shows positive staining in the nucleus and cell junctions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody (AB121380)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody (AB121380)

ab121380 staining DPEP1/MDP in Paraffin-emebedded Human kidney tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DPEP1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P16444

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPEP1 also known as MDP or dipeptidase 1 functions as a membrane-bound enzyme responsible for hydrolyzing dipeptides. It exhibits a mass of approximately 60 kDa and requires zinc ions for its enzymatic activity. Researchers often find DPEP1 in the kidney where it plays a role in the renal brush border. It is expressed also in other tissues including the intestine and can be found in the lungs and placenta. Its structure allows it to effectively act on a variety of dipeptides primarily those with hydrophobic amino acids.

Biological function summary

DPEP1 is involved in the metabolism of leukotriene and the hydrolysis of cysteinyl leukotrienes. It is a component of the renal proximal tubule and contributes to amino acid transport and homeostasis. Its enzymatic activity modulates the levels of these substrates impacting inflammatory responses. The protein does not function alone; it often forms homomeric structures or interacts with other cellular components to perform its biological duties efficiently.

Pathways

The enzymatic activity of DPEP1 makes it integral to the leukotriene pathway affecting both inflammatory and immune responses. It plays a role in the glutathione metabolism pathway contributing to homeostasis and detoxification processes. DPEP1's activity parallels that of other metabolic enzymes such as gamma-glutamyltransferase 1 (GGT1) which also contributes to glutathione and amino acid metabolism. These pathways showcase how DPEP1's function is tied into broader metabolic and regulatory networks.

DPEP1 is associated with conditions such as acute kidney injury and asthma. Abnormal expression or activity of DPEP1 can lead to distorted cysteinyl leukotriene levels affecting inflammation and tissue remodeling in asthmatic conditions. Additionally its expression patterns in renal tissues make it a potential marker or target in kidney disease contexts. Other proteins such as leukotriene C4 synthase interact with DPEP1 in these disease states illustrating its relevance in pathophysiological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed : 2303490, PubMed : 31442408, PubMed : 32325220, PubMed : 6334084). Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (PubMed : 32325220). Also possesses beta lactamase activity and can hydrolyze the beta-lactam antibiotic imipenem (PubMed : 32325220, PubMed : 6334084).. Independently of its dipeptidase activity, acts as an adhesion receptor for neutrophil recruitment from bloodstream into inflamed lungs and liver.

See full target information DPEP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular diabetology 19:92 PubMed32552684

2020

Accumulation of advanced glycation end products (AGEs) is associated with the severity of aortic stenosis in patients with concomitant type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Kopytek,Michał Ząbczyk,Piotr Mazur,Anetta Undas,Joanna Natorska

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DPEP1/MDP antibody

Immunocytochemistry/ Immunofluorescence - Anti-DPEP1/MDP antibody (AB121380)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody (AB121380)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise