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AB242083

Anti-DPEP1/MDP antibody [EPR22222] - BSA and Azide free

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Rabbit Recombinant Monoclonal DPEP1/MDP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

MDP, RDP, DPEP1, Dipeptidase 1, Beta-lactamase, Dehydropeptidase-I, Microsomal dipeptidase, Renal dipeptidase, hRDP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22222] - BSA and Azide free (AB242083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22222] - BSA and Azide free (AB242083)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling DPEP1/MDP with ab230977 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proximal tubules of human kidney (PMID : 8663190) is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab230977).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22222] - BSA and Azide free (AB242083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22222] - BSA and Azide free (AB242083)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP1/MDP with ab230977 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human testis is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab230977).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22222] - BSA and Azide free (AB242083)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22222] - BSA and Azide free (AB242083)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DPEP1/MDP with ab230977 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of human colon cancer (PMID : 28413640; PMID 23839495) is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab230977).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22222

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab242083 is the carrier-free version of ab230977.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPEP1 also known as MDP or dipeptidase 1 functions as a membrane-bound enzyme responsible for hydrolyzing dipeptides. It exhibits a mass of approximately 60 kDa and requires zinc ions for its enzymatic activity. Researchers often find DPEP1 in the kidney where it plays a role in the renal brush border. It is expressed also in other tissues including the intestine and can be found in the lungs and placenta. Its structure allows it to effectively act on a variety of dipeptides primarily those with hydrophobic amino acids.
Biological function summary

DPEP1 is involved in the metabolism of leukotriene and the hydrolysis of cysteinyl leukotrienes. It is a component of the renal proximal tubule and contributes to amino acid transport and homeostasis. Its enzymatic activity modulates the levels of these substrates impacting inflammatory responses. The protein does not function alone; it often forms homomeric structures or interacts with other cellular components to perform its biological duties efficiently.

Pathways

The enzymatic activity of DPEP1 makes it integral to the leukotriene pathway affecting both inflammatory and immune responses. It plays a role in the glutathione metabolism pathway contributing to homeostasis and detoxification processes. DPEP1's activity parallels that of other metabolic enzymes such as gamma-glutamyltransferase 1 (GGT1) which also contributes to glutathione and amino acid metabolism. These pathways showcase how DPEP1's function is tied into broader metabolic and regulatory networks.

DPEP1 is associated with conditions such as acute kidney injury and asthma. Abnormal expression or activity of DPEP1 can lead to distorted cysteinyl leukotriene levels affecting inflammation and tissue remodeling in asthmatic conditions. Additionally its expression patterns in renal tissues make it a potential marker or target in kidney disease contexts. Other proteins such as leukotriene C4 synthase interact with DPEP1 in these disease states illustrating its relevance in pathophysiological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed : 2303490, PubMed : 31442408, PubMed : 32325220, PubMed : 6334084). Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (PubMed : 32325220). Also possesses beta lactamase activity and can hydrolyze the beta-lactam antibiotic imipenem (PubMed : 32325220, PubMed : 6334084).. Independently of its dipeptidase activity, acts as an adhesion receptor for neutrophil recruitment from bloodstream into inflamed lungs and liver.
See full target information DPEP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com