AB235451

Anti-DPEP3 antibody [EPR21862]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal DPEP3 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

UNQ834/PRO1772, DPEP3, Dipeptidase 3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (AB235451)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (AB235451)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining on human germ cells but not Leydig cells (arrow) or Sertoli cells (arrowhead) (PMID : 21724266) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (AB235451)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on tumor epithelial cells of one case human breast cancer is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Please note that DPEP3 was not detected in every breast cancer tissue sample tested due to the heterogeneity of expression.

Supplier Data

Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] (AB235451)

DPEP3 was immunoprecipitated from 0.35 mg of human testis lysate with ab235451 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235451 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Human testis lysate 10 μg (Input).
Lane 2 : ab235451 IP in human testis lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab235451 in human testis lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] (ab235451)

Predicted band size: 54 kDa

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-DPEP3 antibody [EPR21862] (AB235451)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DPEP3 antibody [EPR21862] (ab235451) at 1/1000 dilution

All lanes:

Human testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 54 kDa

Observed band size: 75 kDa

false

Exposure time: 48s

Carrier free

Anti-DPEP3 antibody [EPR21862] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21862

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidase 3 (DPEP3) also known as MBD-2 or membrane-bound dipeptidase 3 is an enzyme that performs important roles in hydrolyzing dipeptides. DPEP3 has a molecular weight of approximately 52 kDa. It belongs to the dipeptidase family and is often found in the membranes of the kidney and testis where it contributes to peptide metabolism. As a metalloprotease DPEP3 requires a metal ion as a cofactor for its catalytic activity.

Biological function summary

DPEP3 contributes to diverse physiological processes in the body. This enzyme often exists as a part of a multi-protein complex facilitating the breakdown of dietary peptides and the renal processing of glutathione and leukotriene D4. Through its enzymatic action DPEP3 helps in managing extracellular matrix homeostasis and detoxifying a wide range of substances that circulate in the body.

Pathways

DPEP3 influences several metabolic pathways by engaging in the breakdown of dipeptides. Of particular note DPEP3 functions within the leukotriene metabolism and glutathione metabolism pathways. In leukotriene metabolism enzymes such as leukotriene A4 hydrolase collaborate with DPEP3 for the conversion of leukotrienes into biologically active lipid mediators. In the glutathione metabolism pathway DPEP3's activity is augmented by its interaction with glutathione S-transferases.

DPEP3 shows relevance to conditions like renal dysfunctions and allergic responses. The enzyme's role in metabolizing leukotrienes links it to asthma and other allergic conditions where dysregulated leukotriene pathways play a part. Furthermore changes in DPEP3 expression might be observed alongside alterations in the activity of glutathione S-transferases which could impact renal health and lead to disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem (PubMed : 32325220). The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide (PubMed : 32325220). A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance (PubMed : 32325220).

See full target information DPEP3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:8778 PubMed40082523

2025

Probiotic Bifidobacterium bifidum strains desialylate MUC13 and increase intestinal epithelial barrier function.

Applications

Unspecified application

Species

Unspecified reactive species

Celia Segui-Perez,Liane Z X Huang,Fernanda L Paganelli,Elke Lievens,Karin Strijbis

Cell death and differentiation 32:1336-1352 PubMed40021897

2025

The Trim32-DPEP2 axis is an inflammatory switch in macrophages during intestinal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyan Zhan,Huisheng Liang,Zhuoqi Zhao,Liya Pan,Jing Li,Yuyun Chen,Zhoulonglong Xie,Zhilong Yan,Ying Xiang,Wenxue Liu,Li Hong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DPEP3 antibody [EPR21862]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (AB235451)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (AB235451)

Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] (AB235451)

Western blot - Anti-DPEP3 antibody [EPR21862] (AB235451)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise