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AB238646

Anti-DPEP3 antibody [EPR21862] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DPEP3 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNQ834/PRO1772, DPEP3, Dipeptidase 3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (AB238646)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (AB238646)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining on human germ cells but not Leydig cells (arrow) or Sertoli cells (arrowhead) (PMID : 21724266) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (AB238646)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (AB238646)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on tumor epithelial cells of one case human breast cancer is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Please note that DPEP3 was not detected in every breast cancer tissue sample tested due to the heterogeneity of expression.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (AB238646)
  • IP

Supplier Data

Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (AB238646)

DPEP3 was immunoprecipitated from 0.35 mg of human testis lysate with ab235451 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235451 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Human testis lysate 10 μg (Input).
Lane 2 : ab235451 IP in human testis lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab235451 in human testis lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

All lanes:

Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] (<a href='/en-us/products/primary-antibodies/dpep3-antibody-epr21862-ab235451'>ab235451</a>)

Predicted band size: 54 kDa

Observed band size: 75 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21862

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab238646 is the carrier-free version of ab235451.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidase 3 (DPEP3) also known as MBD-2 or membrane-bound dipeptidase 3 is an enzyme that performs important roles in hydrolyzing dipeptides. DPEP3 has a molecular weight of approximately 52 kDa. It belongs to the dipeptidase family and is often found in the membranes of the kidney and testis where it contributes to peptide metabolism. As a metalloprotease DPEP3 requires a metal ion as a cofactor for its catalytic activity.
Biological function summary

DPEP3 contributes to diverse physiological processes in the body. This enzyme often exists as a part of a multi-protein complex facilitating the breakdown of dietary peptides and the renal processing of glutathione and leukotriene D4. Through its enzymatic action DPEP3 helps in managing extracellular matrix homeostasis and detoxifying a wide range of substances that circulate in the body.

Pathways

DPEP3 influences several metabolic pathways by engaging in the breakdown of dipeptides. Of particular note DPEP3 functions within the leukotriene metabolism and glutathione metabolism pathways. In leukotriene metabolism enzymes such as leukotriene A4 hydrolase collaborate with DPEP3 for the conversion of leukotrienes into biologically active lipid mediators. In the glutathione metabolism pathway DPEP3's activity is augmented by its interaction with glutathione S-transferases.

DPEP3 shows relevance to conditions like renal dysfunctions and allergic responses. The enzyme's role in metabolizing leukotrienes links it to asthma and other allergic conditions where dysregulated leukotriene pathways play a part. Furthermore changes in DPEP3 expression might be observed alongside alterations in the activity of glutathione S-transferases which could impact renal health and lead to disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem (PubMed : 32325220). The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide (PubMed : 32325220). A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance (PubMed : 32325220).
See full target information DPEP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:882 PubMed38057853

2023

GATA6-AS1 suppresses epithelial-mesenchymal transition of pancreatic cancer under hypoxia through regulating SNAI1 mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhui Zhou,Xinyi Zhou,Qiwen Ben,Ningning Liu,Jiahui Wang,Yongpeng Zhai,Yichen Bao,Lin Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com