AB128149

Anti-DPF2/REQ antibody

Name: Anti-DPF2/REQ antibody (ab128149) | Abcam
Brand: Abcam Limited
SKU: AB128149
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal DPF2/REQ antibody. Suitable for IP and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DPF2 aa 100-200.

View Alternative Names

BAF45D, REQ, UBID4, DPF2, Zinc finger protein ubi-d4, Apoptosis response zinc finger protein, BRG1-associated factor 45D, Protein requiem

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Immunoprecipitation - Anti-DPF2/REQ antibody (AB128149)

Detection of DPF2/REQ in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab128149 at 6 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DPF2/REQ antibody (ab128149)

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human DPF2 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q92785

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Goat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPF2 also known as REQ is a protein that plays a role in chromatin remodeling an important process in gene expression regulation. The molecular weight of DPF2 is approximately 48 kDa. This protein is ubiquitously expressed in various tissues indicating its broad functional roles across different cell types. DPF2 participates actively in the modification of chromatin structure facilitating accessibility of DNA for transcription factors and other regulatory proteins.

Biological function summary

DPF2 acts as a component of the BAF complex which is a multi-subunit chromatin remodeling complex. The BAF complex modifies the structure of chromatin to regulate gene transcription affecting gene expression outcomes. DPF2 helps recruit other important proteins to the BAF complex contributing to the control of gene transcription dynamics during cell differentiation and development. Its function is essential in maintaining proper chromatin state which is important for cellular identity and function.

Pathways

DPF2 interacts significantly within the Wnt and Notch signaling pathways. In the Wnt pathway DPF2 contributes to the regulation of genes involved in cell proliferation differentiation and apoptosis. This protein also interfaces with related pathways by influencing the activity of other proteins such as beta-catenin in the Wnt pathway. In the Notch signaling pathway DPF2 modulates gene expression by affecting chromatin structure which influences cellular processes like proliferation and differentiation.

DPF2 has correlations with leukemia and other cancers. Abnormal expression or mutations of DPF2 possibly affect the balance of chromatin remodeling leading to misregulation of genes that control cell growth and survival. This protein potentially interacts with proteins such as MLL found in mixed lineage leukemia linking DPF2 to pathways that when dysregulated result in oncogenic transformation. Understanding DPF2's role in these disorders might offer novel therapeutic targets or diagnostic markers in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed : 27775714, PubMed : 28533407). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed : 28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed : 20460684).

See full target information DPF2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9614 PubMed39511190

2024

The BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain.

Applications

Unspecified application

Species

Unspecified reactive species

Kwangbeom Hyun,Jihye Ahn,Hyoungmin Kim,Jihyun Kim,Yong-In Kim,Hee-Sung Park,Robert G Roeder,J Eugene Lee,Jaehoon Kim

Frontiers in microbiology 14:1330109 PubMed38156016

2023

Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in .

Applications

Unspecified application

Species

Unspecified reactive species

Petra Spidlova,Eliska Sokolova,Pavla Pavlik

eLife 11: PubMed35551737

2022

Inositol polyphosphate multikinase physically binds to the SWI/SNF complex and modulates BRG1 occupancy in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoon Beon,Sungwook Han,Hyeokjun Yang,Seung Eun Park,Kwangbeom Hyun,Song-Yi Lee,Hyun-Woo Rhee,Jeong Kon Seo,Jaehoon Kim,Seyun Kim,Daeyoup Lee

View all publications

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Product promise

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Anti-DPF2/REQ antibody

Immunoprecipitation - Anti-DPF2/REQ antibody (AB128149)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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