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Rabbit Recombinant Monoclonal DPF2/REQ antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (AB232327), expandable thumbnail
  • Western blot - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (AB232327), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (AB232327), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (AB232327), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (AB232327), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed:27775714, PubMed:28533407). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed:28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed:20460684).

Alternative names

BAF45D, REQ, UBID4, DPF2, Zinc finger protein ubi-d4, Apoptosis response zinc finger protein, BRG1-associated factor 45D, Protein requiem

Recommended products

Rabbit Recombinant Monoclonal DPF2/REQ antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR9206(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab232327 is the carrier-free version of Anti-DPF2/REQ antibody [EPR9206(B)] ab134942.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DPF2 also known as REQ is a protein that plays a role in chromatin remodeling an important process in gene expression regulation. The molecular weight of DPF2 is approximately 48 kDa. This protein is ubiquitously expressed in various tissues indicating its broad functional roles across different cell types. DPF2 participates actively in the modification of chromatin structure facilitating accessibility of DNA for transcription factors and other regulatory proteins.

Biological function summary

DPF2 acts as a component of the BAF complex which is a multi-subunit chromatin remodeling complex. The BAF complex modifies the structure of chromatin to regulate gene transcription affecting gene expression outcomes. DPF2 helps recruit other important proteins to the BAF complex contributing to the control of gene transcription dynamics during cell differentiation and development. Its function is essential in maintaining proper chromatin state which is important for cellular identity and function.

Pathways

DPF2 interacts significantly within the Wnt and Notch signaling pathways. In the Wnt pathway DPF2 contributes to the regulation of genes involved in cell proliferation differentiation and apoptosis. This protein also interfaces with related pathways by influencing the activity of other proteins such as beta-catenin in the Wnt pathway. In the Notch signaling pathway DPF2 modulates gene expression by affecting chromatin structure which influences cellular processes like proliferation and differentiation.

Associated diseases and disorders

DPF2 has correlations with leukemia and other cancers. Abnormal expression or mutations of DPF2 possibly affect the balance of chromatin remodeling leading to misregulation of genes that control cell growth and survival. This protein potentially interacts with proteins such as MLL found in mixed lineage leukemia linking DPF2 to pathways that when dysregulated result in oncogenic transformation. Understanding DPF2's role in these disorders might offer novel therapeutic targets or diagnostic markers in oncology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Immunoprecipitation - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 (purified) at 1/100 dilution (2μg) immunoprecipitating DPF2/REQ in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate.

    Lane 1 (input): HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 10ug
    Lane 2 (+): Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 + HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

    All lanes: Immunoprecipitation - Anti-DPF2/REQ antibody [EPR9206(B)] (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942)

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

  • Western blot - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Western blot - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 observed at 50 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

    Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 was shown to react with DPF2/REQ in wild-type A431 cells in western blot. Loss of signal was observed when DPF2 knockout sample was used. Wild-type and DPF2 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-DPF2/REQ antibody [EPR9206(B)] (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942) at 1/1000 dilution

    Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: DPF2 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human DPF2 knockout A-431 cell line (Human DPF2 knockout A-431 cell line ab270473)

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 44 kDa

    Observed band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DPF2/REQ with purified Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 at 1/200 dilution (8.3μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, a Goat anti-rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling DPF2/REQ with purified Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebral cortex tissue sections labeling DPF2/REQ with purified Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue sections labeling DPF2/REQ with purified Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling DPF2/REQ with purified Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human kidney tissue, labelling DPF2/REQ using unpurified Anti-DPF2/REQ antibody [EPR9206(B)] ab134942 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-DPF2/REQ antibody [EPR9206(B)] ab134942).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327), expandable thumbnail

    OI-RD Scanning - Anti-DPF2/REQ antibody [EPR9206(B)] - BSA and Azide free (ab232327)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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