Rabbit Polyclonal DPF3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DPF3 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DPF3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q92784
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. Isoform 2. Acts as a regulator of myogenesis in cooperation with HDGFL2 (PubMed:32459350). Mediates the interaction of HDGFL2 with the BAF complex (PubMed:32459350). HDGFL2-DPF3a activate myogenic genes by increasing chromatin accessibility through recruitment of SMARCA4/BRG1/BAF190A (ATPase subunit of the BAF complex) to myogenic gene promoters (PubMed:32459350).
Alternative names
BAF45C, CERD4, DPF3, Zinc finger protein DPF3, BRG1-associated factor 45C, Zinc finger protein cer-d4
Recommended products
Rabbit Polyclonal DPF3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DPF3 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DPF3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q92784
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Keep as concentrated solution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DPF3 also known as double PHD fingers 3 or BAF45C is a protein with a mass of approximately 44 kDa. It functions in chromatin remodeling and regulates transcription by altering DNA packaging. DPF3 expression varies but is found in tissues such as the heart and skeletal muscle. The protein contains two PHD finger domains that interact with histones and enable binding to specific sites on the chromatin.
- Biological function summary
DPF3 plays a significant role in muscle differentiation and development. It forms part of the BAF (SWI/SNF) chromatin remodeling complex which regulates gene expression by modifying the structure of chromatin allowing transcription machinery to access DNA more effectively. DPF3 acts in response to signaling pathways that induce muscle cell differentiation contributing to the transcriptional activation of muscle-specific genes.
- Pathways
Chromatin remodeling processes and muscle development pathways heavily involve DPF3. The protein links to the myogenic regulatory pathways where it interacts with other components like MYOD1 which is essential for muscle cell lineage determination. Additionally DPF3 affects the Wnt signaling pathway which is important in various developmental processes and coordinates cellular responses required for muscle growth.
- Associated diseases and disorders
DPF3 has connections to congenital heart diseases and certain types of cardiomyopathy. Mutations and altered expression levels in DPF3 affect heart muscle function linking it to proteins such as NKX2-5 another transcription factor critical for heart development. DPF3's interaction with these proteins can lead to malformations in cardiac structure and function emphasizing its importance in genomic integrity and organogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-DPF3 antibody (ab127703)
10% SDS PAGE
All lanes: Western blot - Anti-DPF3 antibody (ab127703) at 1/500 dilution
All lanes: IMR32 whole cell lysate at 30 µg
Predicted band size: 43 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com