Rabbit Polyclonal DPH4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DNAJC24 aa 1-100.
Images
Publications
Filter by
- Cell death & disease 13:4902022DNAJC24 is a potential therapeutic target in hepatocellular carcinoma through affecting ammonia metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangtao Li,Yuchao He,Hui Liu,Dongming Liu,Lu Chen,Yi Luo,Liwei Chen,Lisha Qi,Yun Wang,Yingying Wang,Yu Wang,Linlin Zhan,Ning Zhang,Xiaolin Zhu,Tianqiang Song,Hua GuoPubMed 35606363
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DNAJC24 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P3W2
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Stimulates the ATPase activity of several Hsp70-type chaperones. This ability is enhanced by iron-binding. The iron-bound form is redox-active and can function as electron carrier. Plays a role in the diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2) which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).
Alternative names
DPH4, ZCSL3, DNAJC24, DnaJ homolog subfamily C member 24, CSL-type zinc finger-containing protein 3, Diphthamide biosynthesis protein 4
Recommended products
Rabbit Polyclonal DPH4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DNAJC24 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DNAJC24 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P3W2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DPH4 also known as diphthamide biosynthesis protein 4 plays an important role in diphthamide synthesis a unique post-translational modification of a histidine residue in eukaryotic elongation factor 2 (eEF2). DPH4 has a molecular mass of approximately 43 kDa. The expression of DPH4 appears predominantly in the cytoplasm across various tissues. Its function supports the activity of eEF2 by stabilizing ribosome translocation during protein synthesis.
- Biological function summary
DPH4 facilitates the final step in diphthamide formation an essential modification for eEF2 functionality. This protein participates in a complex that includes other DPH proteins such as DPH1 DPH2 and DPH5 which work together to transfer and refine the diphthamide group. DPH4's role in this complex is critical for modifying eEF2 a factor necessary for effective protein translation and cellular function.
- Pathways
The diphthamide biosynthesis pathway relies heavily on DPH4's activity. This pathway connects to the protein synthesis pathway emphasizing DPH4's contribution to overall cellular growth and maintenance. Additionally its interaction with eEF2 and other DPH proteins highlights DPH4's integration in translation regulation influencing how cells synthesize proteins under varying conditions.
- Associated diseases and disorders
DPH4's role in diphthamide modification links to susceptibility to bacterial toxins such as those produced by Corynebacterium diphtheriae which must modify eEF2 to exert pathological effects. Mutations or malfunctions in the diphthamide biosynthesis pathway may also associate with carcinomas due to disruptions in protein synthesis. The interaction with eEF2 and other pathway components suggests that DPH4 dysfunction could potentially influence tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-DPH4 antibody (ab246925)
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling DPH4 using DPH4 at 4 μg/ml (green) in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPH4 antibody (ab246925)
Paraffin-embedded human duodenum tissue stained for DPH4 using ab246925 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-DPH4 antibody (ab246925)
All lanes: Western blot - Anti-DPH4 antibody (ab246925) at 0.4 µg/mL
Lane 1: Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2: DPH4 overexpression HEK-293T cell lysate
Predicted band size: 17 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com