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AB246925

Anti-DPH4 antibody

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(2 Publications)

Rabbit Polyclonal DPH4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DNAJC24 aa 1-100.

View Alternative Names

DPH4, ZCSL3, DNAJC24, DnaJ homolog subfamily C member 24, CSL-type zinc finger-containing protein 3, Diphthamide biosynthesis protein 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPH4 antibody (AB246925)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPH4 antibody (AB246925)

Paraffin-embedded human duodenum tissue stained for DPH4 using ab246925 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DPH4 antibody (AB246925)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DPH4 antibody (AB246925)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling DPH4 using DPH4 at 4 μg/ml (green) in ICC/IF.

Western blot - Anti-DPH4 antibody (AB246925)
  • WB

Unknown

Western blot - Anti-DPH4 antibody (AB246925)

All lanes:

Western blot - Anti-DPH4 antibody (ab246925) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

DPH4 overexpression HEK-293T cell lysate

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DNAJC24 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q6P3W2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPH4 also known as diphthamide biosynthesis protein 4 plays an important role in diphthamide synthesis a unique post-translational modification of a histidine residue in eukaryotic elongation factor 2 (eEF2). DPH4 has a molecular mass of approximately 43 kDa. The expression of DPH4 appears predominantly in the cytoplasm across various tissues. Its function supports the activity of eEF2 by stabilizing ribosome translocation during protein synthesis.
Biological function summary

DPH4 facilitates the final step in diphthamide formation an essential modification for eEF2 functionality. This protein participates in a complex that includes other DPH proteins such as DPH1 DPH2 and DPH5 which work together to transfer and refine the diphthamide group. DPH4's role in this complex is critical for modifying eEF2 a factor necessary for effective protein translation and cellular function.

Pathways

The diphthamide biosynthesis pathway relies heavily on DPH4's activity. This pathway connects to the protein synthesis pathway emphasizing DPH4's contribution to overall cellular growth and maintenance. Additionally its interaction with eEF2 and other DPH proteins highlights DPH4's integration in translation regulation influencing how cells synthesize proteins under varying conditions.

DPH4's role in diphthamide modification links to susceptibility to bacterial toxins such as those produced by Corynebacterium diphtheriae which must modify eEF2 to exert pathological effects. Mutations or malfunctions in the diphthamide biosynthesis pathway may also associate with carcinomas due to disruptions in protein synthesis. The interaction with eEF2 and other pathway components suggests that DPH4 dysfunction could potentially influence tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates the ATPase activity of several Hsp70-type chaperones. This ability is enhanced by iron-binding. The iron-bound form is redox-active and can function as electron carrier. Plays a role in the diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2) which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).
See full target information DNAJC24
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:279 PubMed40713574

2025

DNAJC24 suppresses breast cancer malignancy and serves as a prognostic biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Meng,Wei Liu,Yulong Yang,Xiaorui Wang,Linlin Zhan,Yi Luo,Liwei Chen,Yu Wang,Guangtao Li,Yehui Shi,Yuchao He,Zhongsheng Tong,Hua Guo

Cell death & disease 13:490 PubMed35606363

2022

DNAJC24 is a potential therapeutic target in hepatocellular carcinoma through affecting ammonia metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Guangtao Li,Yuchao He,Hui Liu,Dongming Liu,Lu Chen,Yi Luo,Liwei Chen,Lisha Qi,Yun Wang,Yingying Wang,Yu Wang,Linlin Zhan,Ning Zhang,Xiaolin Zhu,Tianqiang Song,Hua Guo
View all publications
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