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AB113686

Anti-DPM1 antibody [5C5A7]

3

(1 Review)

|

(9 Publications)

Mouse Monoclonal DPM1 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 9 publications.

View Alternative Names

SED3, YPR183W, P9705.3, DPM1, Dolichol-phosphate mannosyltransferase, Dolichol-phosphate mannose synthase, Dolichyl-phosphate beta-D-mannosyltransferase, Mannose-P-dolichol synthase, DPM synthase, MPD synthase

1 Images
Western blot - Anti-DPM1 antibody [5C5A7] (AB113686)
  • WB

Unknown

Western blot - Anti-DPM1 antibody [5C5A7] (AB113686)

All lanes:

Western blot - Anti-DPM1 antibody [5C5A7] (ab113686)

All lanes:

Yeast whole cell lysate at 15 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5C5A7

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPM1 also known as dolichyl-phosphate beta-D-mannosyltransferase subunit 1 is an important component of the dolichol-phosphate mannose synthase complex. It has a molecular mass of approximately 29 kDa. DPM1 is localized in the endoplasmic reticulum membrane where it plays a significant role in the synthesis of glycosylphosphatidylinositol (GPI) anchors and protein glycosylation.
Biological function summary

This protein is essential in transferring mannose from GDP-mannose to dolichol phosphate a reaction that synthesizes dolichol-phosphate mannose (DPM). DPM is an important donor substrate in the glycan assembly pathways. DPM1 does not have a transmembrane domain unlike other components of the DPM synthase complex. Association with other complex subunits like DPM2 and DPM3 stabilize its structure and function within the cell ensuring glycosylation processes proceed correctly.

Pathways

Glycosylation is one of the fundamental pathways DPM1 participates in. Its role in glycosylation links it to the protein N-glycosylation pathway and GPI anchor biosynthesis pathway. DPM1 interacts with proteins such as DPM2 in the synthase complex to facilitate the conversion process necessary for glycosylation of proteins and lipids impacting various cellular processes like cell-cell adhesion and protein folding.

DPM1 molecule relates to congenital disorders of glycosylation (CDG) particularly CDG-Ie. Mutations in the gene encoding DPM1 can disrupt enzymatic activity leading to issues in glycosylation which manifest in multisystem disorders. The connection of DPM1 to these disorders often involves deficiencies in dolichol-phosphate mannose production. Similarly defects in associated proteins like DPM2 can compound the glycosylation defects exacerbating disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.
See full target information DPM1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Methods in enzymology 700:77-104 PubMed38971613

2024

Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Israel Juarez-Contreras,Itay Budin

The Journal of biological chemistry 300:105496 PubMed38013088

2023

Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Itay Budin

The FEBS journal 288:3285-3299 PubMed33283454

2020

Phosphatidylserine flux into mitochondria unveiled by organelle-targeted Escherichia coli phosphatidylserine synthase PssA.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroya Shiino,Shiina Furuta,Rieko Kojima,Keisuke Kimura,Toshiya Endo,Yasushi Tamura

eLife 9: PubMed31909711

2020

The genetic basis of aneuploidy tolerance in wild yeast.

Applications

Unspecified application

Species

Unspecified reactive species

James Hose,Leah E Escalante,Katie J Clowers,H Auguste Dutcher,DeElegant Robinson,Venera Bouriakov,Joshua J Coon,Evgenia Shishkova,Audrey P Gasch

Nature communications 10:1432 PubMed30926815

2019

Phosphatidylethanolamine made in the inner mitochondrial membrane is essential for yeast cytochrome bc complex function.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Calzada,Erica Avery,Pingdewinde N Sam,Arnab Modak,Chunyan Wang,J Michael McCaffery,Xianlin Han,Nathan N Alder,Steven M Claypool

Nature communications 8:15832 PubMed28607491

2017

An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion.

Applications

WB

Species

Saccharomyces cerevisiae S288C

Laetitia Cavellini,Julie Meurisse,Justin Findinier,Zoi Erpapazoglou,Naïma Belgareh-Touzé,Allan M Weissman,Mickael M Cohen

Biology open 5:689-97 PubMed27142334

2016

Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration.

Applications

WB

Species

Unspecified reactive species

Jessica A Simpkins,Kirby E Rickel,Marianna Madeo,Bethany A Ahlers,Gabriel B Carlisle,Heidi J Nelson,Andrew L Cardillo,Emily A Weber,Peter F Vitiello,David A Pearce,Seasson P Vitiello

Molecular biology of the cell 26:726-39 PubMed25540432

2014

Seipin performs dissectible functions in promoting lipid droplet biogenesis and regulating droplet morphology.

Applications

Unspecified application

Species

Unspecified reactive species

Bethany R Cartwright,Derk D Binns,Christopher L Hilton,Sungwon Han,Qiang Gao,Joel M Goodman

Aging 5:551-74 PubMed23924582

2013

Mitochondrial membrane lipidome defines yeast longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Beach,Vincent R Richard,Anna Leonov,Michelle T Burstein,Simon D Bourque,Olivia Koupaki,Mylène Juneau,Rachel Feldman,Tatiana Iouk,Vladimir I Titorenko
View all publications
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Product promise

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