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AB217127

Anti-DPP3 antibody

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(1 Publication)

Rabbit Polyclonal DPP3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DPP3 aa 650-700.

View Alternative Names

Dipeptidyl peptidase 3, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase III, Enkephalinase B, DPP III, DPP3

2 Images
Immunoprecipitation - Anti-DPP3 antibody (AB217127)
  • IP

Supplier Data

Immunoprecipitation - Anti-DPP3 antibody (AB217127)

Detection of DPP3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217127 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab217127 was used at 1μg/ml. Lane 2 Control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DPP3 antibody (ab217127)

Predicted band size: 83 kDa

true

Exposure time: 10s

Western blot - Anti-DPP3 antibody (AB217127)
  • WB

Supplier Data

Western blot - Anti-DPP3 antibody (AB217127)

Lysis buffer : NETN

All lanes:

Western blot - Anti-DPP3 antibody (ab217127) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 15 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 15 µg

Lane 4:

TCMK1 whole cell lysate at 15 µg

Lane 5:

NIH 3T3 whole cell lysate at 15 µg

Predicted band size: 83 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DPP3 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

Q9NY33

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab217127 was affinity purified using an epitope specific to DPP3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein DPP3 short for Dipeptidyl Peptidase 3 is an enzyme with a mass of approximately 82 kDa. It belongs to the M49 family of metallopeptidases and is widely expressed in human tissues including kidney heart liver and brain. This enzyme has the function of cleaving dipeptides from the N-terminus of polypeptides. Alternative names for DPP3 include DPP III and dipeptidyl peptidase III. By breaking down peptides DPP3 plays an important role in proteolysis.
Biological function summary

DPP3 influences several physiological processes. It is reported to participate in the degradation of enkephalins which are neuropeptides involved in regulating pain. DPP3 does not form part of a larger complex but operates independently within cells to execute its functions. By degrading its substrates DPP3 helps modulate peptide signaling pathways and maintain cellular homeostasis.

Pathways

DPP3 has significant involvement in the enkephalin degradation pathway which is essential in pain modulation and stress responses. It also plays a part in the ubiquitin-proteasome pathway facilitating the degradation of damaged or misfolded proteins. DPP3 interacts with proteins such as the ubiquitin-conjugating enzyme influencing protein turnover processes.

DPP3 links closely to cardiovascular diseases and cancer. Elevated levels of DPP3 are observed in heart failure where it may disrupt normal peptide balance and contribute to disease progression. Additionally its relationship with proteins such as angiotensin-converting enzyme (ACE) may affect blood pressure regulation. In certain cancers DPP3 expression changes can influence tumor growth by altering peptide-mediated signaling pathways with its interactions suggesting potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves and degrades bioactive peptides, including angiotensin, Leu-enkephalin and Met-enkephalin (PubMed : 1515063, PubMed : 3233187). Also cleaves Arg-Arg-beta-naphthylamide (in vitro) (PubMed : 11209758, PubMed : 3233187, PubMed : 9425109).
See full target information DPP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 74:37 PubMed39738657

2025

Targeting OAS3 for reversing M2d infiltration and restoring anti-tumor immunity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaopeng Zhang,Ximo Xu,Kundong Zhang,Changzheng Lei,Yitian Xu,Pengshan Zhang,Yuan Zhang,Haitao Gu,Chen Huang,Zhengjun Qiu
View all publications
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