Anti-DPP3 antibody [EPR9020(B)] - BSA and Azide free

• WB

Lab

Western blot - Anti-DPP3 antibody [EPR9020(B)] - BSA and Azide free (AB248606)

Blocking and diluting buffer concentrations : 5% NFDM/TBST.

Exposure time : Lane 1 : 8 seconds, Lanes 2-3 : 6 seconds.

In lane 1, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

This data was developed using ab133671, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DPP3 antibody [EPR9020(B)] (<a href='/en-us/products/primary-antibodies/dpp3-antibody-epr9020b-ab133671'>ab133671</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 3:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 83 kDa

Observed band size: 82 kDa

false

• WB

Unknown

Western blot - Anti-DPP3 antibody [EPR9020(B)] - BSA and Azide free (AB248606)

This data was developed using ab133671, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DPP3 antibody [EPR9020(B)] - BSA and Azide free (ab248606) at 1/10000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

U87 MG cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 83 kDa

false