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Rabbit Recombinant Monoclonal DPP3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Predicted
Not recommended
Not recommended
Rat
Not recommended
Predicted
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Cleaves and degrades bioactive peptides, including angiotensin, Leu-enkephalin and Met-enkephalin (PubMed:3233187, PubMed:1515063). Also cleaves Arg-Arg-beta-naphthylamide (in vitro) (PubMed:9425109, PubMed:3233187, PubMed:11209758).

Alternative names

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Rabbit Recombinant Monoclonal DPP3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR9021(B)

Purity

Tissue culture supernatant

Dissociation constant

6.6 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

DPP3 influences several physiological processes. It is reported to participate in the degradation of enkephalins which are neuropeptides involved in regulating pain. DPP3 does not form part of a larger complex but operates independently within cells to execute its functions. By degrading its substrates DPP3 helps modulate peptide signaling pathways and maintain cellular homeostasis.

Mechanical summary

The protein DPP3 short for Dipeptidyl Peptidase 3 is an enzyme with a mass of approximately 82 kDa. It belongs to the M49 family of metallopeptidases and is widely expressed in human tissues including kidney heart liver and brain. This enzyme has the function of cleaving dipeptides from the N-terminus of polypeptides. Alternative names for DPP3 include DPP III and dipeptidyl peptidase III. By breaking down peptides DPP3 plays an important role in proteolysis.

Pathway

DPP3 has significant involvement in the enkephalin degradation pathway which is essential in pain modulation and stress responses. It also plays a part in the ubiquitin-proteasome pathway facilitating the degradation of damaged or misfolded proteins. DPP3 interacts with proteins such as the ubiquitin-conjugating enzyme influencing protein turnover processes.

Disease

DPP3 links closely to cardiovascular diseases and cancer. Elevated levels of DPP3 are observed in heart failure where it may disrupt normal peptide balance and contribute to disease progression. Additionally its relationship with proteins such as angiotensin-converting enzyme (ACE) may affect blood pressure regulation. In certain cancers DPP3 expression changes can influence tumor growth by altering peptide-mediated signaling pathways with its interactions suggesting potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-DPP3 antibody [EPR9021(B)] (ab133735), expandable thumbnail

    Western blot - Anti-DPP3 antibody [EPR9021(B)] (ab133735)

    All lanes: Western blot - Anti-DPP3 antibody [EPR9021(B)] (AB133735) at 1/10000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Lane 4: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 83 kDa

  • OI-RD Scanning - Anti-DPP3 antibody [EPR9021(B)] (ab133735), expandable thumbnail

    OI-RD Scanning - Anti-DPP3 antibody [EPR9021(B)] (ab133735)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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