AB119346

Anti-DPP4 antibody [236.3]

Name: Anti-DPP4 antibody [236.3] (ab119346) | Abcam
Brand: Abcam Limited
SKU: AB119346
Price: 535 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal DPP4 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Dpp4.

View Alternative Names

CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV

5 Images

Flow Cytometry - Anti-DPP4 antibody [236.3] (AB119346)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DPP4 antibody [236.3] (AB119346)

Flow cytometry analysis of CD26 in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD26 monoclonal antibody (ab119346) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [236.3] (AB119346)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [236.3] (AB119346)

ab119346 labelling CD26 (green) in the cytoplasm of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 20 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [236.3] (AB119346)

ab119346 labelling CD26 (green) in the cytoplasm of H-4-II-E cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 20 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [236.3] (AB119346)

ab119346 labelling CD26 (green) in the cytoplasm of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 20 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DPP4 antibody [236.3] (AB119346)

Flow cytometry analysis of CD26 in PC12 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD26 monoclonal antibody (ab119346) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

236.3

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Rat Dpp4. The exact immunogen used to generate this antibody is proprietary information.

P14740

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.

Biological function summary

DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.

Pathways

DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.

DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed : 10593948, PubMed : 16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed : 18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed : 17549790). Also acts as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed : 10570924, PubMed : 16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed : 10593948).. (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.

See full target information DPP4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 10:826093 PubMed35372314

2022

Application of 3D Hepatic Plate-Like Liver Model for Statin-Induced Hepatotoxicity Evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiecheng Xu,Daogang Pan,Wei Liao,Zhidong Jia,Mingxin Pan,Jun Weng,Xu Han,Shao Li,Yang Li,Kangyan Liang,Shuqin Zhou,Qing Peng,Yi Gao

Advances in wound care 11:455-465 PubMed34521222

2021

A Novel Xenograft Model Demonstrates Human Fibroblast Behavior During Skin Wound Repair and Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mimi R Borrelli,Abra H Shen,Michelle Griffin,Shamik Mascharak,Sandeep Adem,Nestor M Diaz Deleon,Ledibabari Mildred Ngaage,Michael T Longaker,Derrick C Wan,Hermann Peter Lorenz

Viruses 11: PubMed31491938

2019

A Fusion Peptide in the Spike Protein of MERS Coronavirus.

Applications

Unspecified application

Species

Unspecified reactive species

Entedar A J Alsaadi,Benjamin W Neuman,Ian M Jones

Oncotarget 10:3559-3575 PubMed31191826

2019

Interleukin 1 is a key driver of inflammatory bowel disease-demonstration in a murine IL-1Ra knockout model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha H Dosh,Nicola Jordan-Mahy,Christopher Sammon,Christine Le Maitre

Scientific reports 8:3490 PubMed29472575

2018

Glial cell type-specific changes in spinal dipeptidyl peptidase 4 expression and effects of its inhibitors in inflammatory and neuropatic pain.

Applications

IHC-P

Species

Unspecified reactive species

Kornél Király,Márk Kozsurek,Erika Lukácsi,Benjamin Barta,Alán Alpár,Tamás Balázsa,Csaba Fekete,Judit Szabon,Zsuzsanna Helyes,Kata Bölcskei,Valéria Tékus,Zsuzsanna E Tóth,Károly Pap,Gábor Gerber,Zita Puskár

Oncology letters 10:34-42 PubMed26170973

2015

Expression levels of seprase/FAPα and DPPIV/CD26 in epithelial ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhen Zhang,Liwei Xu,Xiaoling Wang,Beibei Sun,Juan Ding

The Biochemical journal 273 ( Pt 3):497-502 PubMed1705112

1991

A Fischer rat substrain deficient in dipeptidyl peptidase IV activity makes normal steady-state RNA levels and an altered protein. Use as a liver-cell transplantation model.

Applications

IP, IHC

Species

Unspecified reactive species, Unspecified reactive species

N L Thompson,D C Hixson,H Callanan,M Panzica,D Flanagan,R A Faris,W J Hong,S Hartel-Schenk,D Doyle

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DPP4 antibody [236.3]

Flow Cytometry - Anti-DPP4 antibody [236.3] (AB119346)

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [236.3] (AB119346)

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [236.3] (AB119346)

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [236.3] (AB119346)

Flow Cytometry - Anti-DPP4 antibody [236.3] (AB119346)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise