AB187048

Anti-DPP4 antibody [EPR18215]

Name: Anti-DPP4 antibody [EPR18215] (ab187048) | Abcam
Brand: Abcam Limited
SKU: AB187048
Price: 230 USD
Availability: InStock
Rating: 5 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(25 Publications)

Anti-DPP4 antibody [EPR18215] (ab187048) is a rabbit monoclonal antibody detecting DPP4 in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

CD26, Cd26, Dpp4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, T-cell activation antigen CD26, Thymocyte-activating molecule, DPP IV, THAM

5 Images

Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat liver tissue labeling CD26 with ab187048 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID : 23361237). The nuclear counter stain is DAPI (blue)
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Antigen retrieval : Heated Tris-EDTA (pH9.0) (ab94681).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse liver tissue labeling CD26 with ab187048 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID : 23361237). The nuclear counter stain is DAPI (blue)
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Antigen retrieval : Heated Tris-EDTA (pH9.0) (ab94681).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of rat liver is observed (PMID : 23361237). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of mouse liver is observed (PMID : 23361237). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Western blot - Anti-DPP4 antibody [EPR18215] (AB187048)

Blocking and dilution buffer : 5% NFDM/TBST

Exposure times.

Lanes 1, 3, 4 : 30 seconds

Lane 2 : 20 seconds

Lane 5 : 10 seconds

Lane 6 : 15 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID : 25589660).

All lanes:

Western blot - Anti-DPP4 antibody [EPR18215] (ab187048) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

Mouse thymus lysate at 20 µg

Lane 4:

Rat thymus lysate at 20 µg

Lane 5:

Rat liver lysate at 20 µg

Lane 6:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 88 kDa

Observed band size: 110 kDa

false

Carrier free

Anti-DPP4 antibody [EPR18215] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-DPP4 antibody [EPR18215]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-DPP4 antibody [EPR18215]
Biotin

Biotin Anti-DPP4 antibody [EPR18215]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18215

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200 - 1/1000", "IHCFr-species-notes": "Rat: use at 1/200Mouse: use at 1/1000", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200 - 1/1000", "IHCFr-species-notes": "Rat: use at 1/200Mouse: use at 1/1000", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-DPP4 antibody [EPR18215] (ab187048) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

What is the molecular weight of DPP4?
Anti-DPP4 [EPR18215] (ab187048) specifically detects a band for DPP4 (UniProt: P28843) at a molecular weight of 88kDa.

Trusted by the scientific community
Anti-DPP4 [EPR18215] (ab187048) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR18215] also available for your convenience: ab187048, Carrier free - ab232221, Biotin - ab317766, Alexa Fluor® 647 - ab322420, Alexa Fluor® 488 - ab322423

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.

Biological function summary

DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.

Pathways

DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.

DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Also acts as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

See full target information Dpp4

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2025:2379872 PubMed41069927

2025

Transcriptomics and Proteomics Reveal That TLPW Acupuncture Ameliorates Proteinuria in Diabetic Kidney Disease Model Rats by Suppressing Epithelial-to-Mesenchymal Transition via the DPP4/SDF-1/TGF-/Smad Signalling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Ji,Jiya Sun,Zihao Zhuang,Yunming Xiao,Shipian Li,Zhilong Zhang,Xu Wang,Xinju Li

iScience 28:113489 PubMed41019369

2025

Therapeutic potential of 5HT2CR in alleviating spasticity and pain via modulation of motor neurons and interneurons in spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Han Gong,Zuliyaer Talifu,Yu-Zhe Sun,Zhao-Ming Yue,Xiao-Xia Du,Liang-Jie Du

Genes & development : PubMed40930990

2025

regulates early postnatal DPP4 preadipocyte pool expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Irem Altun,Khanh Ho Diep Vo,Safal Walia,Xiaocheng Yan,Inderjeet Singh,Ruth Karlina,Viktorian Miok,Lingru Kang,Valentin Kenneth Reichenbach,Andreas Israel,Dominik Lutter,Fabiana Perrochi,Siegfried Ussar

Journal of nanobiotechnology 23:506 PubMed40660260

2025

Human adipose-derived mesenchymal stem cell-derived exosomes induce epithelial remodeling and anti-scar healing revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Fu,Jun-Ling Xie,Xing-Liao Zhang,Guang-Ming Xie,Xin-Min Zhang,Yao-Ting Han,Meng-Meng Xu,Jing Zhang,Jun Zhang

Nature communications 16:749 PubMed39820504

2025

A CD26 tendon stem progenitor cell population contributes to tendon repair and heterotopic ossification.

Applications

Unspecified application

Species

Unspecified reactive species

Siwen Chen,Yingxin Lin,Hao Yang,Zihao Li,Sifang Li,Dongying Chen,Wenjun Hao,Shuai Zhang,Hua Chao,Jingyu Zhang,Jianru Wang,Zemin Li,Xiang Li,Zhongping Zhan,Hui Liu

Molecules and cells 47:100159 PubMed39577744

2024

Dipeptidyl peptidase 4 as an injury-responsive protein in the mouse sciatic nerve.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsoo Oh,Yongcheol Cho

Scientific reports 14:15099 PubMed38956264

2024

Boiling histotripsy exhibits anti-fibrotic effects in animal models of liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chanmin Joung,Jeongmin Heo,Ki Joo Pahk,Kisoo Pahk

Diabetes, metabolic syndrome and obesity : targets and therapy 17:55-73 PubMed38192494

2024

Claudin-2 Mediates the Proximal Tubular Epithelial Cell-Fibroblast Crosstalk via Paracrine CTGF.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Guo,Mingwen Jiao,Yuying Cui,Pingjiang Li,Jinming Yao,Jianjun Dong,Lin Liao

Histology and histopathology :18676 PubMed37997936

2023

WITHDRAWN: Ethyl acetate extracted from the chinese medicinal plant Caesalpinia sappan L alleviates gustatory response in diabetes mellitus rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Cao,Li Cui,Wen Yang

Endokrynologia Polska 74:190-196 PubMed37039492

2023

Sodium glucose cotransporter-2-inhibitor dapagliflozin improves nonalcoholic fatty liver disease by ameliorating dipeptidyl-peptidase-4 protein expression in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Ming Zhao,Cheng-Qiang Li,Yu-Man Sun,Jin-Yang Fan,Nan Wu,Ya-Nan Sun,Xin-Yi Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DPP4 antibody [EPR18215]

Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (AB187048)

Western blot - Anti-DPP4 antibody [EPR18215] (AB187048)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

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Product protocols

Target data

Publications (25)

Product promise