AB222716

Anti-DPP4 antibody [EPR21945]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal DPP4 antibody. Suitable for IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Recombinant fragment - Mouse samples. Cited in 4 publications.

View Alternative Names

CD26, Cd26, Dpp4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, T-cell activation antigen CD26, Thymocyte-activating molecule, DPP IV, THAM

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [EPR21945] (AB222716)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [EPR21945] (AB222716)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse PBMC (mouse peripheral blood mononuclear cells) cells labeling CD26 with ab222716 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in mouse PBMCs. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry - Anti-DPP4 antibody [EPR21945] (AB222716)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DPP4 antibody [EPR21945] (AB222716)

Flow cytometric analysis of mouse PBMCs (mouse peripheral blood mononuclear cells) labeling CD26 with ab222716 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Supplier Data

Immunoprecipitation - Anti-DPP4 antibody [EPR21945] (AB222716)

CD26 was immunoprecipitated from 0.35mg of mouse lung tissue lysate with ab222716 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Mouse lung tissue lysate 10 μg (Input).

Lane 2 : ab222716 IP in mouse lung tissue lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab222716 in mouse lung tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-DPP4 antibody [EPR21945] (ab222716)

Predicted band size: 88 kDa

Observed band size: 110 kDa

false

ELISA - Anti-DPP4 antibody [EPR21945] (AB222716)

ELISA

Supplier Data

ELISA - Anti-DPP4 antibody [EPR21945] (AB222716)

ELISA analysis of Mouse Dpp4 recombinant protein at 1000 ng/mL with ab222716. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-DPP4 antibody [EPR21945] (AB222716)

Blocking/Dilution : 5% NFDM/TBST

Exposure.

Lane 1 : 147 seconds, Lane 2 : 37 seconds

All lanes:

Western blot - Anti-DPP4 antibody [EPR21945] (ab222716) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 88 kDa

Observed band size: 110 kDa

true

Carrier free

Anti-DPP4 antibody [EPR21945] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21945

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ELISA, Flow Cyt, WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.

Biological function summary

DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.

Pathways

DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.

DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Also acts as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

See full target information Dpp4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 17:1267-1279 PubMed38434584

2024

BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei He,Danyang Jing,Shen Zhao,Meili Duan

Cancer cell 40:1392-1406.e7 PubMed36270275

2022

Multiomic analysis reveals conservation of cancer-associated fibroblast phenotypes across species and tissue of origin.

Applications

Unspecified application

Species

Unspecified reactive species

Deshka S Foster,Michael Januszyk,Daniel Delitto,Kathryn E Yost,Michelle Griffin,Jason Guo,Nicholas Guardino,Andrea E Delitto,Malini Chinta,Austin R Burcham,Alan T Nguyen,Khristian E Bauer-Rowe,Ashley L Titan,Ankit Salhotra,R Ellen Jones,Oscar da Silva,Hunter G Lindsay,Charlotte E Berry,Kellen Chen,Dominic Henn,Shamik Mascharak,Heather E Talbott,Alexia Kim,Fatemeh Nosrati,Dharshan Sivaraj,R Chase Ransom,Michael Matthews,Anum Khan,Dhananjay Wagh,John Coller,Geoffrey C Gurtner,Derrick C Wan,Irene L Wapnir,Howard Y Chang,Jeffrey A Norton,Michael T Longaker

BMC immunology 23:34 PubMed35778692

2022

The impact of HIV infection on the frequencies, function, spatial localization and heterogeneity of T follicular regulatory cells (TFRs) within human lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Bongiwe Mahlobo,Faatima Laher,Werner Smidt,Funsho Ogunshola,Trevor Khaba,Thandeka Nkosi,Anele Mbatha,Thandekile Ngubane,Krista Dong,Ismail Jajbhay,Johan Pansegrouw,Zaza M Ndhlovu

Nature communications 11:4061 PubMed32792541

2020

Elucidating the fundamental fibrotic processes driving abdominal adhesion formation.

Applications

Unspecified application

Species

Unspecified reactive species

Deshka S Foster,Clement D Marshall,Gunsagar S Gulati,Malini S Chinta,Alan Nguyen,Ankit Salhotra,R Ellen Jones,Austin Burcham,Tristan Lerbs,Lu Cui,Megan E King,Ashley L Titan,R Chase Ransom,Anoop Manjunath,Michael S Hu,Charles P Blackshear,Shamik Mascharak,Alessandra L Moore,Jeffrey A Norton,Cindy J Kin,Andrew A Shelton,Michael Januszyk,Geoffrey C Gurtner,Gerlinde Wernig,Michael T Longaker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DPP4 antibody [EPR21945]

Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [EPR21945] (AB222716)

Flow Cytometry - Anti-DPP4 antibody [EPR21945] (AB222716)

Immunoprecipitation - Anti-DPP4 antibody [EPR21945] (AB222716)

ELISA - Anti-DPP4 antibody [EPR21945] (AB222716)

Western blot - Anti-DPP4 antibody [EPR21945] (AB222716)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise