Skip to main content

Rabbit Recombinant Monoclonal DPP6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-DPP6 antibody [EPR15944-24] (AB198506), expandable thumbnail
  • Western blot - Anti-DPP6 antibody [EPR15944-24] (AB198506), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (AB198506), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (AB198506), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (AB198506), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Promotes cell surface expression of the potassium channel KCND2 (PubMed:15454437, PubMed:19441798). Modulates the activity and gating characteristics of the potassium channel KCND2 (PubMed:18364354). Has no dipeptidyl aminopeptidase activity (PubMed:15476821, PubMed:8103397).

Alternative names

A-type potassium channel modulatory protein DPP6, DPPX, Dipeptidyl aminopeptidase-like protein 6, Dipeptidyl aminopeptidase-related protein, Dipeptidyl peptidase 6, Dipeptidyl peptidase IV-like protein, Dipeptidyl peptidase VI, DPP VI, DPP6

Recommended products

Rabbit Recombinant Monoclonal DPP6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15944-24
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein dipeptidyl peptidase-like protein 6 (DPP6) also known as DPPX plays a significant role in the regulation of neuronal excitability. It is a single-pass type II membrane protein with a molecular mass of approximately 93 kDa. DPP6 is mainly expressed in the brain where it is found in various regions such as the cerebellum and hippocampus. This protein is not a conventional dipeptidyl peptidase as it lacks the catalytic activity found in other members of its family. Instead DPP6 associates with other proteins to influence their functions.

Biological function summary

DPP6 interacts with voltage-gated potassium channels specifically the Kv4 family to form a multiprotein complex. This complex plays an essential role in controlling the electrical signaling properties of neurons by regulating the fast inactivation and recovery of the potassium current. The modulation of these channels impacts neuronal firing frequency and synaptic integration implicating DPP6 in diverse neurophysiological processes.

Pathways

DPP6 is involved in pathways related to synaptic transmission and cortical excitability. It prominently influences the MAPK signaling pathway as its interaction with Kv4 channels affects the downstream signaling cascades. Additionally DPP6 relates to the Wnt signaling pathway which involves several proteins like ß-catenin impacting cell communication and neuronal development. These associations highlight the protein's involvement in neuronal function and plasticity.

Associated diseases and disorders

Dysfunction in DPP6 is linked to neurological conditions such as epilepsy and ataxia. Alterations in DPP6 expression or mutations can lead to impaired neuron excitability contributing to these disorders. DPP6 also connects with other proteins like KCND2 through its role in epilepsy influencing how these conditions manifest at a molecular level. Understanding DPP6's involvement in these diseases assists in identifying potential therapeutic targets for intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506), expandable thumbnail

    Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 12575952

    All lanes: Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506) at 1/5000 dilution

    Lane 1: Rat brain lysate at 10 µg

    Lane 2: Human fetal brain lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 98 kDa

    Observed band size: 115 kDa

    Exposure time: 3min

  • Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506), expandable thumbnail

    Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 12575952.

    All lanes: Western blot - Anti-DPP6 antibody [EPR15944-24] (ab198506) at 1/1000 dilution

    All lanes: Mouse brain lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 98 kDa

    Observed band size: 115 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling DPP6 with ab198506 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cell membrane and cytoplasm staining of Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DPP6 with ab198506 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. No staining of Human liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling DPP6 with ab198506 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cell membrane and cytoplasm staining of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP6 antibody [EPR15944-24] (ab198506)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling DPP6 with ab198506 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. No staining of Mouse liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com