Rabbit Polyclonal DPP8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DPP8 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Cell biology international 46:213-2212021Dipeptidyl peptidase-8 induces sorafenib resistance via binding with c-Rel to mediate NF-κB signaling in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Fu Liu,Yuan-Kang Xie,Bai-Yin Zhong,Jian-Hong Zhang,Cai-Xin Song,Yu-Wen Liu,Yan Yang,Bin-Hui XiePubMed 34719075
- The journal of gene medicine 20:e30562018Targeting dipeptidyl peptidase 8 genes inhibits proliferation, migration and invasion by inhibition of cyclin D1 and MMP2MMP9 signal pathway in cervical cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYurou Chen,Fulin Liu,Kejia Wu,Wanrong Wu,Hanshu Wu,Wei ZhangPubMed 30225951
Key facts
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Tris glycine, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DPP8 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q6V1X1
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 (PubMed:11012666, PubMed:12534281, PubMed:12662155, PubMed:15039077, PubMed:15664838, PubMed:20536396, PubMed:29382749). Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8 (PubMed:27820798, PubMed:29967349, PubMed:32796818). Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation (PubMed:33731929, PubMed:33731932, PubMed:34019797). The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (By similarity).
Alternative names
DPRP1, MSTP097, MSTP135, MSTP141, DPP8, Dipeptidyl peptidase 8, DP8, Dipeptidyl peptidase IV-related protein 1, Dipeptidyl peptidase VIII, Prolyl dipeptidase DPP8, DPRP-1, DPP VIII
Recommended products
Rabbit Polyclonal DPP8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DPP8 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Tris glycine, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DPP8 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q6V1X1
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dipeptidyl peptidase 8 also known as DPP8 is a serine protease enzyme that cleaves x-proline dipeptides from the N-termini of peptides. It has a molecular mass of around 88 kDa and shares structural similarities with DPP4. DPP8 is expressed in various tissues including the brain liver and skin but shows a notably high expression in immune cells. The enzyme is involved in regulating various physiological processes depending on its tissue distribution.
- Biological function summary
DPP8 seems to play roles in modulating immune responses and cell proliferation. It functions independently and is not typically part of a larger protein complex. Studies suggest a connection between DPP8 and cellular processes such as apoptosis and inflammation although the exact biological roles require more detailed investigation.
- Pathways
The peptidase activity of DPP8 integrates within metabolic and immune system pathways. In the context of metabolism it relates closely to proteins like DPP4 which influences glucose homeostasis. In immune pathways DPP8 regulates the processing of peptides affecting immune cell communication and activity sharing roles with other immune-related proteases.
- Associated diseases and disorders
DPP8 exhibits connections with inflammatory and metabolic conditions. For example its dysregulation may relate to inflammatory bowel disease (IBD) possibly affecting inflammatory cytokine release. DPP8's function parallels with DPP4 linking it indirectly to disorders like type 2 diabetes where DPP4 inhibitors are a therapeutic angle. Further exploration of DPP8's roles could indicate potential as a therapeutic target in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-DPP8 antibody (ab113226)
7.5% SDS PAGE.
All lanes: Western blot - Anti-DPP8 antibody (ab113226) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 103 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (ab113226)
ab113226 at 1/200 dilution staining DPP8 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP8 antibody (ab113226)
ab113226, at 10µg/ml, staining DPP8 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP8 antibody (ab113226)
ab113226, at 10µg/ml, staining DPP8 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
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