AB113226

Anti-DPP8 antibody

Name: Anti-DPP8 antibody (ab113226) | Abcam
Brand: Abcam Limited
SKU: AB113226
Price: 630 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal DPP8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DPP8 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DPRP1, MSTP097, MSTP135, MSTP141, DPP8, Dipeptidyl peptidase 8, DP8, Dipeptidyl peptidase IV-related protein 1, Dipeptidyl peptidase VIII, Prolyl dipeptidase DPP8, DPRP-1, DPP VIII

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (AB113226)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (AB113226)

ab113226 at 1/200 dilution staining DPP8 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP8 antibody (AB113226)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP8 antibody (AB113226)

ab113226, at 10µg/ml, staining DPP8 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP8 antibody (AB113226)

ab113226, at 10µg/ml, staining DPP8 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Unknown

Western blot - Anti-DPP8 antibody (AB113226)

7.5% SDS PAGE.

All lanes:

Western blot - Anti-DPP8 antibody (ab113226) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 103 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human DPP8 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q6V1X1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: Tris glycine, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 8 also known as DPP8 is a serine protease enzyme that cleaves x-proline dipeptides from the N-termini of peptides. It has a molecular mass of around 88 kDa and shares structural similarities with DPP4. DPP8 is expressed in various tissues including the brain liver and skin but shows a notably high expression in immune cells. The enzyme is involved in regulating various physiological processes depending on its tissue distribution.

Biological function summary

DPP8 seems to play roles in modulating immune responses and cell proliferation. It functions independently and is not typically part of a larger protein complex. Studies suggest a connection between DPP8 and cellular processes such as apoptosis and inflammation although the exact biological roles require more detailed investigation.

Pathways

The peptidase activity of DPP8 integrates within metabolic and immune system pathways. In the context of metabolism it relates closely to proteins like DPP4 which influences glucose homeostasis. In immune pathways DPP8 regulates the processing of peptides affecting immune cell communication and activity sharing roles with other immune-related proteases.

DPP8 exhibits connections with inflammatory and metabolic conditions. For example its dysregulation may relate to inflammatory bowel disease (IBD) possibly affecting inflammatory cytokine release. DPP8's function parallels with DPP4 linking it indirectly to disorders like type 2 diabetes where DPP4 inhibitors are a therapeutic angle. Further exploration of DPP8's roles could indicate potential as a therapeutic target in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 (PubMed : 11012666, PubMed : 12534281, PubMed : 12662155, PubMed : 15039077, PubMed : 15664838, PubMed : 20536396, PubMed : 29382749). Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8 (PubMed : 27820798, PubMed : 29967349, PubMed : 32796818). Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation (PubMed : 33731929, PubMed : 33731932, PubMed : 34019797). The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (By similarity).

See full target information DPP8

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 46:213-221 PubMed34719075

2021

Dipeptidyl peptidase-8 induces sorafenib resistance via binding with c-Rel to mediate NF-κB signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Fu Liu,Yuan-Kang Xie,Bai-Yin Zhong,Jian-Hong Zhang,Cai-Xin Song,Yu-Wen Liu,Yan Yang,Bin-Hui Xie

Science advances 5:eaax2166 PubMed31579823

2019

Disruptive variants of associate with autism and interfere with neuronal development and synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Guo,Ying Li,Lu Shen,Tianyun Wang,Xiangbin Jia,Lijuan Liu,Tao Xu,Mengzhu Ou,Kendra Hoekzema,Huidan Wu,Madelyn A Gillentine,Cenying Liu,Hailun Ni,Pengwei Peng,Rongjuan Zhao,Yu Zhang,Chanika Phornphutkul,Alexander P A Stegmann,Carlos E Prada,Robert J Hopkin,Joseph T Shieh,Kirsty McWalter,Kristin G Monaghan,Peter M van Hasselt,Koen van Gassen,Ting Bai,Min Long,Lin Han,Yingting Quan,Meilin Chen,Yaowen Zhang,Kuokuo Li,Qiumeng Zhang,Jieqiong Tan,Tengfei Zhu,Yaning Liu,Nan Pang,Jing Peng,Daryl A Scott,Seema R Lalani,Mahshid Azamian,Grazia M S Mancini,Darius J Adams,Malin Kvarnung,Anna Lindstrand,Ann Nordgren,Jonathan Pevsner,Ikeoluwa A Osei-Owusu,Corrado Romano,Giuseppe Calabrese,Ornella Galesi,Jozef Gecz,Eric Haan,Judith Ranells,Melissa Racobaldo,Magnus Nordenskjold,Suneeta Madan-Khetarpal,Jessica Sebastian,Susie Ball,Xiaobing Zou,Jingping Zhao,Zhengmao Hu,Fan Xia,Pengfei Liu,Jill A Rosenfeld,Bert B A de Vries,Raphael A Bernier,Zhi-Qing David Xu,Honghui Li,Wei Xie,Robert B Hufnagel,Evan E Eichler,Kun Xia

The journal of gene medicine 20:e3056 PubMed30225951

2018

Targeting dipeptidyl peptidase 8 genes inhibits proliferation, migration and invasion by inhibition of cyclin D1 and MMP2MMP9 signal pathway in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yurou Chen,Fulin Liu,Kejia Wu,Wanrong Wu,Hanshu Wu,Wei Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DPP8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (AB113226)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP8 antibody (AB113226)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP8 antibody (AB113226)

Western blot - Anti-DPP8 antibody (AB113226)

Key facts

Host species

Clonality

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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