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AB226334

Anti-DPP9 antibody

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(1 Publication)

Rabbit Polyclonal DPP9 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DPP9 aa 750-850.

View Alternative Names

DPRP2, DPP9, Dipeptidyl peptidase 9, DP9, Dipeptidyl peptidase IV-related protein 2, Dipeptidyl peptidase IX, Dipeptidyl peptidase-like protein 9, DPRP-2, DPP IX, DPLP9

1 Images
Immunoprecipitation - Anti-DPP9 antibody (AB226334)
  • IP

Supplier Data

Immunoprecipitation - Anti-DPP9 antibody (AB226334)

DPP9 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226334 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226334 at 0.4 μg/ml.

Lane 1 : ab226334 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-DPP9 antibody (ab226334)

Predicted band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human DPP9 aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

Q86TI2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab226334 was affinity purified using an epitope specific to DPP9 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 9 (DPP9) is an intracellular serine protease belonging to the S9B family primarily recognized for its enzymatic function of cleaving dipeptides from polypeptides. It is known alternatively as DPPIV-like protease with a molecular weight of approximately 92 kDa. DPP9 is expressed in multiple tissues with higher levels seen in the liver kidney and lung. Its role is critical for processing proteins influencing their maturation and function.
Biological function summary

DPP9 acts in several processes beyond simple protease activity. It modulates antigen presentation and immune responses. It functions mainly intracellularly and is not typically secreted. While DPP9 does not form stable complexes with other proteins it does interact dynamically with several cellular proteins to perform its roles. Its enzyme activity can regulate peptide hormones and chemokines implicating it in immune modulation.

Pathways

DPP9 plays roles in the regulation of insulin signaling and immune system pathways. It modulates the activity of proteins like fibroblast growth factor (FGF) and is directly involved in the processing of bioactive peptides related to the immune response. DPP9 shares functional traits with DPP8 another member of the dipeptidyl peptidase family which also cleaves similar substrates and participates in overlapping pathways.

DPP9 is implicated in certain cancers and inflammatory diseases. For instance its dysregulation correlates with hepatocellular carcinoma. High DPP9 expression is also linked to inflammatory diseases where immune system alterations are pronounced. DPP4 a related enzyme also participates in these conditions highlighting potential therapeutic intervention points targeting both DPP9 and DPP4.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 (PubMed : 12662155, PubMed : 16475979, PubMed : 19667070, PubMed : 29382749, PubMed : 30291141, PubMed : 33731929, PubMed : 36112693). Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8 (PubMed : 27820798, PubMed : 29967349, PubMed : 30291141, PubMed : 31525884, PubMed : 32796818, PubMed : 36112693, PubMed : 36357533). Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation (PubMed : 33731929, PubMed : 33731932, PubMed : 34019797). The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP9, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (PubMed : 33731929).
See full target information DPP9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal, genetic engineering & biotechnology 20:77 PubMed35608724

2022

Expression and characterization of hemagglutinin-neuraminidase protein from Newcastle disease virus in Bacillus subtilis WB800.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammadreza Shafaati,Masoud Ghorbani,Minoo Mahmoodi,Mostafa Ebadi,Reza Jalalirad
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