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AB154642

Anti-Dppa4 antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal DPPA4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DPPA4 aa 1 to C-terminus.

View Alternative Names

Developmental pluripotency-associated protein 4, DPPA4

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Dppa4 antibody (AB154642)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Dppa4 antibody (AB154642)

Immunofluorescent analysis of paraformaldehyde-fixed Human ESC labeling Dppa4 with ab154642 at a 1/400 dilution.
The lower image has been stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dppa4 antibody (AB154642)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dppa4 antibody (AB154642)

Immunohistochemical analysis of paraffin embedded H661 (human cell line) xenograft tissue labelling Dppa4 with ab154642 at a 1/100 dilution.

Western blot - Anti-Dppa4 antibody (AB154642)
  • WB

Unknown

Western blot - Anti-Dppa4 antibody (AB154642)

12% SDS PAGE

All lanes:

Western blot - Anti-Dppa4 antibody (ab154642) at 1/500 dilution

All lanes:

Human ESC whole cell lysate at 20 µg

Predicted band size: 34 kDa

false

Western blot - Anti-Dppa4 antibody (AB154642)
  • WB

Unknown

Western blot - Anti-Dppa4 antibody (AB154642)

12% SDS PAGE

All lanes:

Western blot - Anti-Dppa4 antibody (ab154642) at 1/500 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg/mL

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DPPA4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7L190

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternatively Tris-EDTA buffer (pH8.0) may be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
See full target information DPPA4

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38474345

2024

DPPA2/4 Promote the Pluripotency and Proliferation of Bovine Extended Pluripotent Stem Cells by Upregulating the PI3K/AKT/GSK3β/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Fang,Jing Wang,Guangbo Liu,Burong Qu,Jian Chunyu,Wenqiang Xu,Jinzhu Xiang,Xueling Li

Scientific reports 8:12187 PubMed30111816

2018

Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata).

Applications

Unspecified application

Species

Unspecified reactive species

Risako Nakai,Mari Ohnuki,Kota Kuroki,Haruka Ito,Hirohisa Hirai,Ryunosuke Kitajima,Toko Fujimoto,Masato Nakagawa,Wolfgang Enard,Masanori Imamura

Developmental biology 400:43-58 PubMed25624265

2015

Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development.

Applications

IHC-P

Species

Common marmoset

Zachary Yu-Ching Lin,Takamasa Hirano,Shinsuke Shibata,Naomi M Seki,Ryunosuke Kitajima,Ayako Sedohara,Mikiko C Siomi,Erika Sasaki,Haruhiko Siomi,Masanori Imamura,Hideyuki Okano
View all publications

Product promise

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