AB216869

Anti-DPS1 antibody [EPR20843]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DPS1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

DPS1, TPRT, PDSS1, All trans-polyprenyl-diphosphate synthase PDSS1, All-trans-decaprenyl-diphosphate synthase subunit 1, Decaprenyl pyrophosphate synthase subunit 1, Decaprenyl-diphosphate synthase subunit 1, Solanesyl-diphosphate synthase subunit 1, Trans-prenyltransferase 1, TPT 1

2 Images

Supplier Data

Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (AB216869)

DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
Lane 2 : ab216869 IP in MCF7 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST
Exposure time : 150 seconds.

All lanes:

Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)

Predicted band size: 46 kDa

Observed band size: 38 kDa

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Supplier Data

Western blot - Anti-DPS1 antibody [EPR20843] (AB216869)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes.

The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

All lanes:

Western blot - Anti-DPS1 antibody [EPR20843] (ab216869) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 6:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 38 kDa

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Carrier free

Anti-DPS1 antibody [EPR20843] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20843

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

collaborative project

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPS1 also known as Damage Protection Protein 1 plays a significant role at the mechanical level by acting as a DNA-binding protein that protects DNA from oxidative damage. The protein has an approximate mass of 20 kDa and it is mainly expressed in stress-responsive tissues including the liver and kidney. DPS1 stabilizes DNA by forming homodimers that bind to the DNA providing a shield against harmful agents like free radicals.

Biological function summary

DPS1 contributes to the cellular response to oxidative stress and is integral in the defense mechanism of cells. It interacts with various antioxidants to minimize cellular damage and is a component of the multiprotein DNA protection complex. This complex ensures that cells maintain genomic integrity under stressful conditions enhancing the survival rate of cells exposed to damaging agents.

Pathways

DPS1 is closely involved in the oxidative stress response pathway and the DNA repair pathway. These pathways are critical for cellular homeostasis and cellular longevity. DPS1 works closely with proteins such as NRF2 and SOD1 to modulate the response to oxidative stress coordinating the reduction of reactive oxygen species and initiation of DNA repair processes.

DPS1 has associations with conditions like cancer and neurodegenerative disorders. In cancer disruptions in DPS1 activity can lead to increased DNA damage accumulation contributing to tumor progression. In neurodegenerative disorders the protein's impaired function can result in heightened oxidative stress and neuronal damage. DPS1 interacts with proteins such as p53 in cancer influencing cellular responses to DNA damage and with proteins like APP in neurodegenerative diseases affecting pathways that mitigate oxidative stress.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heterotetrameric enzyme that catalyzes the condensation of farnesyl diphosphate (FPP), which acts as a primer, and isopentenyl diphosphate (IPP) to produce prenyl diphosphates of varying chain lengths and participates in the determination of the side chain of ubiquinone (PubMed : 16262699). Supplies nona and decaprenyl diphosphate, the precursors for the side chain of the isoprenoid quinones ubiquinone-9 (Q9)and ubiquinone-10 (Q10) respectively (PubMed : 16262699). The enzyme adds isopentenyl diphosphate molecules sequentially to farnesyl diphosphate with trans stereochemistry (PubMed : 16262699).

See full target information PDSS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1678176 PubMed41050673

2025

CXCR1 fate mapping distinguishes cochlear resident and recruited macrophages after acoustic trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Sree Varshini Murali,Andrew R Stothert,Elyssa Pereyra,Lyudmila Batalkina,Tejbeer Kaur

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DPS1 antibody [EPR20843]

Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (AB216869)

Western blot - Anti-DPS1 antibody [EPR20843] (AB216869)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise