Anti-DPS1 antibody [EPR20843] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-DPS1 antibody [EPR20843] - BSA and Azide free (AB233697)

DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
Lane 2 : ab216869 IP in MCF7 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST
Exposure time : 150 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216869).

All lanes:

Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (<a href='/en-us/products/primary-antibodies/dps1-antibody-epr20843-ab216869'>ab216869</a>)

Predicted band size: 46 kDa

Observed band size: 38 kDa

false

• WB

Supplier Data

Western blot - Anti-DPS1 antibody [EPR20843] - BSA and Azide free (AB233697)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes.

The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216869).

All lanes:

Western blot - Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 6:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

false