AB255823

Anti-DPT/TRAMP antibody [EPR22845-83]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal DPT/TRAMP antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

2 Images

Supplier Data

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (AB255823)

Blocking and Dilution Buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate. There is weak expression in human testis, liver and pancreas. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10233760, 8082810).

Exposure time : 125 seconds

All lanes:

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution

Lane 1:

Human testis lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Lane 3:

Human pancreas lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 22 kDa

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Supplier Data

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (AB255823)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10233760 and 8082810).

Negative control : HepG2 (PMID : 19916908).

Exposure time :

Lanes 1-3 : 15 seconds; Lanes 4 : 59 seconds

All lanes:

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution

Lane 1:

Human skin lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Rat skin lysate at 20 µg

Lane 4:

Mouse skin lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22845-83

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPT also known as Dermatopontin is a protein with a molecular mass of approximately 22 kDa. It is expressed in various tissues with high expression levels in the skin heart and lungs. Dermatopontin interacts with extracellular matrix components and influences cell adhesion and matrix organization. The protein plays a role in regulating fibroblast activity and collagen binding making it important in connective tissue structure and function.

Biological function summary

Dermatopontin contributes to maintaining dermal structural integrity and facilitating wound healing. As part of a larger protein interaction network it enhances cellular communication and tissue repair processes. Dermatopontin modulates the bioavailability of TGF-beta which is essential for collagen production and tissue homeostasis. This highlights its presence in skin remodeling and repair mechanisms linking it to new tissue formation.

Pathways

Dermatopontin participates actively in the TGF-beta signaling pathway and the ECM-receptor interaction pathway. These pathways are critical for cellular communication and tissue structuring processes. The protein interacts with Integrins and Fibronectin facilitating cell-to-ECM adhesion and remodeling activities. Consequently Dermatopontin's pathway involvement supports dynamic tissue responses such as healing and functional adaptation to environmental changes.

Dermatopontin shows associations with fibrosis and keloids. These disorders feature aberrant tissue formation and scarring wherein DPT's regulation of fibroblasts and collagen dynamics plays a notable role. Additionally Dermatopontin's interactions with other proteins like Collagen VI and Decorin influence its involvement in these conditions contributing to the understanding of pathological tissue remodeling mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).

See full target information Dermatopontin

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw5133 PubMed40737399

2025

Biomimetic targeted self-adaptive nanodrug for inflammation optimization and AT2 cell modulation in precise ARDS therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Danfeng He,Xilan Li,Zelin Ou,Hong Wang,Zhinan Shou,Li Wang,Zhengwei Mao,Xiaolan Qi,Jun Deng

Cell death & disease 14:106 PubMed36774339

2023

DNMT3a-dermatopontin axis suppresses breast cancer malignancy via inactivating YAP.

Applications

Unspecified application

Species

Unspecified reactive species

Danrong Ye,Yuying Wang,Xiaochong Deng,Xiqian Zhou,Diya Liu,Baian Zhou,Wenfang Zheng,Xuehui Wang,Lin Fang

Journal of Cancer 11:6288-6298 PubMed33033513

2020

Dermatopontin inhibits WNT signaling pathway via CXXC finger protein 4 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shihai Liu,Jing Qiu,Guifang He,Chao Geng,Weitai He,Changchang Liu,Duo Cai,Huazheng Pan,Qingwu Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DPT/TRAMP antibody [EPR22845-83]

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (AB255823)

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (AB255823)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise