JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB256824

Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal DPT/TRAMP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

2 Images
Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (AB256824)
  • WB

Supplier Data

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (AB256824)

Blocking and Dilution Buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate. There is weak expression in human testis, liver and pancreas. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10233760, 8082810).

Exposure time : 125 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255823).

All lanes:

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (<a href='/en-us/products/primary-antibodies/dpt-tramp-antibody-epr22845-83-ab255823'>ab255823</a>) at 1/1000 dilution

Lane 1:

Human testis lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Lane 3:

Human pancreas lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 22 kDa

false

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (AB256824)
  • WB

Supplier Data

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (AB256824)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10233760 and 8082810).

Negative control : HepG2 (PMID : 19916908).

Exposure time :

Lanes 1-3 : 15 seconds; Lanes 4 : 59 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255823).

All lanes:

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (<a href='/en-us/products/primary-antibodies/dpt-tramp-antibody-epr22845-83-ab255823'>ab255823</a>) at 1/1000 dilution

Lane 1:

Human skin lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Rat skin lysate at 20 µg

Lane 4:

Mouse skin lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22845-83

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab256824 is the carrier-free version of ab255823.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPT also known as Dermatopontin is a protein with a molecular mass of approximately 22 kDa. It is expressed in various tissues with high expression levels in the skin heart and lungs. Dermatopontin interacts with extracellular matrix components and influences cell adhesion and matrix organization. The protein plays a role in regulating fibroblast activity and collagen binding making it important in connective tissue structure and function.
Biological function summary

Dermatopontin contributes to maintaining dermal structural integrity and facilitating wound healing. As part of a larger protein interaction network it enhances cellular communication and tissue repair processes. Dermatopontin modulates the bioavailability of TGF-beta which is essential for collagen production and tissue homeostasis. This highlights its presence in skin remodeling and repair mechanisms linking it to new tissue formation.

Pathways

Dermatopontin participates actively in the TGF-beta signaling pathway and the ECM-receptor interaction pathway. These pathways are critical for cellular communication and tissue structuring processes. The protein interacts with Integrins and Fibronectin facilitating cell-to-ECM adhesion and remodeling activities. Consequently Dermatopontin's pathway involvement supports dynamic tissue responses such as healing and functional adaptation to environmental changes.

Dermatopontin shows associations with fibrosis and keloids. These disorders feature aberrant tissue formation and scarring wherein DPT's regulation of fibroblasts and collagen dynamics plays a notable role. Additionally Dermatopontin's interactions with other proteins like Collagen VI and Decorin influence its involvement in these conditions contributing to the understanding of pathological tissue remodeling mechanisms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).
See full target information Dermatopontin
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB221576

Anti-DPT/TRAMP antibody

Immunocytochemistry/ Immunofluorescence - Anti-DPT/TRAMP antibody (AB221576)

  • 3
  • 2
Primary Antibodies

AB230236

Anti-DPT/TRAMP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPT/TRAMP antibody (AB230236)

  • 0
  • 0

Complementary Products

Assay Kits

AB272206

Human DPT ELISA Kit (TRAMP)

Recombinant|SimpleStep

ELISA - Human DPT ELISA Kit (TRAMP) (AB272206)

  • 0
  • 0
Primary Antibodies

AB179810

Anti-LOXL2 antibody [EPR12733] - C-terminal

RabMAb|Recombinant|KO Validated

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (AB179810)

  • 4
  • 6
Primary Antibodies

AB277636

Anti-Decorin antibody [EPR24097-105]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Western blot - Anti-Decorin antibody [EPR24097-105] (AB277636)

  • 0
  • 0
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Secondary Antibodies

AB150129

Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (AB150129)

  • 5
  • 6

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com