JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB205039

Anti-DPYS antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal DPYS antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DPYS aa 100-300.

View Alternative Names

Dihydropyrimidinase, DHP, DHPase, Dihydropyrimidine amidohydrolase, Hydantoinase, DPYS

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPYS antibody (AB205039)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPYS antibody (AB205039)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human fetal pancreas tissue sections labeling DPYS with ab205039 at a 1/100 dilution.

Western blot - Anti-DPYS antibody (AB205039)
  • WB

Supplier Data

Western blot - Anti-DPYS antibody (AB205039)

All lanes:

Western blot - Anti-DPYS antibody (ab205039) at 1/500 dilution

All lanes:

human fetal liver lysate

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DPYS aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q14117

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPYS also known as dihydropyrimidinase is an enzyme involved in the breakdown of pyrimidines specifically converting dihydropyrimidines to N-carbamyl-B-alanine. The mass of DPYS is approximately 56 kDa. This enzyme is present in various tissues most notably in the liver and kidney. DPYS plays a role in the catabolic pathway of nucleic acids facilitating the reuse and recycling of building blocks required for nucleic acid synthesis.
Biological function summary

DPYS functions as part of the larger pyrimidine degradation pathway. It does not act in isolation but may associate with other enzymes responsible for subsequent steps in this pathway. Pyrimidine metabolism is essential for maintaining the nucleotide pool balance in cells aiding cellular replication and repair processes. DPYS ensures efficient conversion of potentially harmful intermediates into products that cells can either reuse or excrete safely.

Pathways

The activity of DPYS is integrated into the pyrimidine catabolic pathway which intersects with the urea cycle. This enzyme works alongside others such as dihydropyrimidine dehydrogenase (DPYD) to facilitate pyrimidine degradation. The interactions between DPYS and DPYD are critical as they control the levels of pyrimidine metabolites in the cell preventing accumulation that could cause cellular stress or damage.

Mutations in the DPYS gene correlate with the rare metabolic disorder called dihydropyrimidinuria. This condition manifests due to the inefficient breakdown of pyrimidines leading to excessive urinary excretion of dihydropyrimidines. Furthermore impaired DPYS activity may have implications in drug metabolism particularly fluoropyrimidine-based cancer therapies as reduced activity can lead to toxicity. DPYD also connects to these conditions through its role in pyrimidine degradation highlighting the interdependence of these enzymes in both regular metabolic function and disease states.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
See full target information DPYS
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Drug metabolism and disposition: the biological fate of chemicals 51:165-173 PubMed36414408

2022

Functional Characterization of 12 Dihydropyrimidinase Allelic Variants in Japanese Individuals for the Prediction of 5-Fluorouracil Treatment-Related Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Eiji Hishinuma,Yoko Narita,Evelyn Marie Gutiérrez Rico,Akiko Ueda,Kai Obuchi,Yoshikazu Tanaka,Sakae Saito,Shu Tadaka,Kengo Kinoshita,Masamitsu Maekawa,Nariyasu Mano,Tomoki Nakayoshi,Akifumi Oda,Noriyasu Hirasawa,Masahiro Hiratsuka

American journal of cancer research 9:1722-1733 PubMed31497353

2019

USP44 suppresses pancreatic cancer progression and overcomes gemcitabine resistance by deubiquitinating FBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Yang,Shikai Zhu,Hongji Yang,Sisi Deng,Ping Fan,Mi Li,Xin Jin
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB121844

Anti-DPYS antibody

Immunocytochemistry/ Immunofluorescence - Anti-DPYS antibody (AB121844)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB40800

Anti-CAD antibody [EP710Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CAD antibody [EP710Y] (AB40800)

  • 0
  • 0
Primary Antibodies

AB195045

Anti-PAX6 antibody [EPR15858]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] (AB195045)

  • 5
  • 9
Primary Antibodies

AB121991

Anti-HENMT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)

  • 0
  • 0
Primary Antibodies

AB177509

Anti-GIGYF1 antibody

Immunoprecipitation - Anti-GIGYF1 antibody (AB177509)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com